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L2023

Leupeptin trifluoroacetate salt

powder, ≥90% (HPLC)

Synonym(s):

Acetyl-Leu-Leu-Arg-al trifluoroacetate salt

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1 mg
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$75.60
5 mg
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$150.00
10 mg
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$262.00
25 mg
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$479.00
50 mg
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$837.00
$711.45

About This Item

Empirical Formula (Hill Notation):
C20H38N6O4 · xC2HF3O2
CAS Number:
Molecular Weight:
426.55 (free base basis)
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.77
MDL number:

$75.60


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Product Name

Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial

biological source

microbial

Quality Segment

assay

≥90% (HPLC)

form

powder

input

general use sample(s)

solubility

H2O: 10 mM (Solutions are stable only a few hours. Stock solutions are stable up to 6 months at −20 °C.)

storage temp.

−20°C

SMILES string

OC(=O)C(F)(F)F.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O

InChI

1S/C20H38N6O4.C2HF3O2/c1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;3-2(4,5)1(6)7/h11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H,6,7)/t15-,16-,17-;/m0./s1

InChI key

WUHGBZVQELGOMH-FRKSIBALSA-N

Biochem/physiol Actions

Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM.
Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)

Analysis Note

Leupeptin gives multiple peaks on HPLC because of the formation of tautomeric isomers in solution. Majority of contaminating peptide is racemized leupeptin.

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This Item
L9783L064962070
description

≥90% (HPLC), microbial

description

microbial, ≥90% (HPLC)

description

microbial, ≥70% (HPLC)

description

>4000 U/mg

biological source

microbial

biological source

microbial

biological source

microbial

biological source

synthetic

assay

≥90% (HPLC)

assay

≥90% (HPLC)

assay

≥70% (HPLC)

assay

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

powder

form

powder

form

powder

form

powder

solubility

H2O: 10 mM (Solutions are stable only a few hours. Stock solutions are stable up to 6 months at −20 °C.)

solubility

water: 50 mg/mL, clear, colorless

solubility

water: 50 mg/mL, clear, colorless to yellow

solubility

H2O: 50 mg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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