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Anti-Luciferase antibody, Mouse monoclonal

Name: Anti-Luciferase antibody, Mouse monoclonal
Brand: SIGMA
Price: 544 USD
Availability: InStock

Enhanced Validation

clone LUC-1, purified from hybridoma cell culture

Synonym(s):

Anti-Luciferase Antibody

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Size/SKU	Availability	Price
0.2 mL	Estimated to ship onOctober 14, 2026fromMILWAUKEE	$544.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.56

MDL number:

MFCD01092407

Conjugate:

unconjugated

Clone:

LUC-1, monoclonal

Application:

ICC, WB

Citations:

$544.00

Estimated to ship onOctober 14, 2026Details

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LUC-1, monoclonal

form

buffered aqueous solution

mol wt

60 kDa

enhanced validation

recombinant expression
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concentration

~2 mg/mL

technique(s)

immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase, immunocytochemistry: suitable, western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Anti-Luciferase antibody, Mouse monoclonal , (mouse IgG1 isotype) is derived from the LUC-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with firefly (Photinus pyralis) luciferase. Luciferase, a reporter protein contains two binding pockets, one pocket for ATP and other one for luciferin substrate. Both the pockets are in close proximity to each other.

The mouse monoclonal Anti-Luciferase antibody recognizes the recombinant luciferase in transfected cells. This antibody provides user with an alternative detection method for luciferase. Since it directly detects the luciferase protein, it is not dependent on either the luciferase activity or the measeurement of rapid reaction kinetics. This also allows for the detection of the luciferase enzyme expression in situ with reproducibility between sample sets.

Application

Anti-Luciferase antibody, Mouse monoclonal has been used in:

immunoblotting
immunocytochemistry
immunohistochemistry

Biochem/physiol Actions

Anti-Luciferase antibody, Mouse monoclonal recognizes recombinant luciferase in transfected eukaryotic (293T) cells.

Luciferase catalyzes a bioluminescent reaction in the presence of substrate luciferin as well as Mg²⁺, oxygen and ATP. The luciferase assay is rapid, sensitive, and unlike the CAT assay, does not require a radioactive substrate. Anti-Luciferase antibody can detect the luciferase enzyme expression in situ, providing a means to study the cellular localization of the signal sequence activation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in a frost-free freezer, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Working Dilution: 1-2 μg/ml

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R5404	M7815	A4471
Sigma-Aldrich L2164 Anti-Luciferase antibody, Mouse monoclonal	Sigma-Aldrich R5404 Monoclonal Anti-Rab9 antibody produced in mouse	Sigma-Aldrich M7815 Anti-MDM2 antibody ,Mouse monoclonal	Sigma-Aldrich A4471 Anti-ARTS antibody, Mouse monoclonal
description clone LUC-1, purified from hybridoma cell culture	description ~2 mg/mL, clone Rab9-29, purified immunoglobulin, buffered aqueous solution	description clone HDM2-323, purified from hybridoma cell culture	description clone ARTS51, purified from hybridoma cell culture
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone LUC-1, monoclonal	clone Rab9-29, monoclonal	clone HDM2-323, monoclonal	clone ARTS51, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
mol wt 60 kDa	mol wt ~23 kDa	mol wt antigen ~90 kDa	mol wt antigen 32 kDa
technique(s) western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase, western blot: suitable, immunocytochemistry: suitable, immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase	technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 2 μg/mL using total cell extract of HeLa cells.	technique(s) indirect ELISA: suitable, western blot: 1-2 μg/mL (whole cell extract of 293T cells transfected with human MDM2), microarray: suitable	technique(s) microarray: suitable, indirect ELISA: suitable, western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS, immunocytochemistry: suitable

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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