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L2164

Anti-Luciferase antibody, Mouse monoclonal

clone LUC-1, purified from hybridoma cell culture

enhanced validation

Enhanced Validation

Synonym(s):

Anti-Luciferase Antibody

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0.2 mL
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$564.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
MFCD01092407
Conjugate:
unconjugated
Clone:
LUC-1, monoclonal
Application:
ICC, WB
Citations:
31

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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LUC-1, monoclonal

form

buffered aqueous solution

mol wt

60 kDa

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase, immunocytochemistry: suitable, western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Luciferase antibody, Mouse monoclonal , (mouse IgG1 isotype) is derived from the LUC-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with firefly (Photinus pyralis) luciferase. Luciferase, a reporter protein contains two binding pockets, one pocket for ATP and other one for luciferin substrate. Both the pockets are in close proximity to each other.
The mouse monoclonal Anti-Luciferase antibody recognizes the recombinant luciferase in transfected cells. This antibody provides user with an alternative detection method for luciferase. Since it directly detects the luciferase protein, it is not dependent on either the luciferase activity or the measeurement of rapid reaction kinetics. This also allows for the detection of the luciferase enzyme expression in situ with reproducibility between sample sets.

Application

Anti-Luciferase antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunohistochemistry

Biochem/physiol Actions

Anti-Luciferase antibody, Mouse monoclonal recognizes recombinant luciferase in transfected eukaryotic (293T) cells.
Luciferase catalyzes a bioluminescent reaction in the presence of substrate luciferin as well as Mg2+, oxygen and ATP. The luciferase assay is rapid, sensitive, and unlike the CAT assay, does not require a radioactive substrate. Anti-Luciferase antibody can detect the luciferase enzyme expression in situ, providing a means to study the cellular localization of the signal sequence activation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in a frost-free freezer, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Working Dilution: 1-2 μg/ml

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
L0159AB3256119113
Anti-Luciferase antibody, Mouse monoclonal clone LUC-1, purified from hybridoma cell culture

Sigma-Aldrich

L2164

Anti-Luciferase antibody, Mouse monoclonal

Anti-Luciferase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

L0159

Anti-Luciferase antibody produced in rabbit

Anti-Luciferase Antibody, Firefly

Sigma-Aldrich

AB3256

Anti-Luciferase Antibody, Firefly

Luciferase Inhibitor I The Luciferase Inhibitor I, also referenced under CAS 352341-26-5, controls the biological activity of Luciferase.

Sigma-Aldrich

119113

Luciferase Inhibitor I

description

clone LUC-1, purified from hybridoma cell culture

description

IgG fraction of antiserum, buffered aqueous solution

description

-

description

The Luciferase Inhibitor I, also referenced under CAS 352341-26-5, controls the biological activity of Luciferase.

clone

LUC-1, monoclonal

clone

polyclonal

clone

polyclonal

clone

-

biological source

mouse

biological source

rabbit

biological source

-

biological source

-

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

purified antibody

antibody form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

solid

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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