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Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse

Name: Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse
Brand: SIGMA
Price: 511.7 USD
Availability: InStock

~2 mg/mL, clone 6E10, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-CMD1T, Anti-Cardiomyopathy, dilated, 1T, included, Anti-Lamina Associated Polypeptide 2, Anti-TMPO, Anti-Thymopoietin

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$602.00 $511.70

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02263594

Conjugate:

unconjugated

Clone:

6E10, monoclonal

Application:

ARR, ICC, WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6E10, monoclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa (ζ isoform), antigen 38 kDa (γ isoform, singlet or doublet), antigen 42 kDa (δ isoform), antigen 45 kDa (ε isoform), antigen 50 kDa (β isoform, singlet or doublet), antigen 76 kDa (α isoform)

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using whole extract of cultured human chronic myelogenous leukemia K562 cells.

isotype

IgG1

UniProt accession no.

P42166

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TMPO(7112)

Description

General description

Monoclonal Anti-LAP2 (TMPO) (mouse IgG1 isotype) is derived from the 6E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide (amino acids 29-50) common to the N-terminus of all TMPOs. Thymopoietin β (TMPOβ)/lamina associated polypeptide 2 (LAP2) is a type II integral protein of the inner nuclear envelope (NE). In mammalian cells, six alternatively spliced TMPO isoforms, designated α, β, β′, γ, δ, ε, and ζ have been isolated and characterized. LAP2/TMPO proteins is highly conserved in mammals and Xenopus. The expression of the various TMPO isoforms is ubiquitous, with higher levels in proliferative tissues. It has four domains: a hydrophilic C-terminus domain, a hydrophobic transmembrane domain, a NE targeting and lamina-binding domain, and a chromatin binding domain. TMPOβ/LAP2 contains two putative p34cdc2 kinase phosphorylation sites.

Immunogen

synthetic peptide corresponding to amino acids 29-50 common to the N-terminus of all human TMPOs (isoforms α, β, γ, δ, ε, and ζ).

Application

Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse may be used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

Thymopoietin β (TMPOβ)/lamina associated polypeptide 2 (LAP2) is putatively involved in functional nuclear architecture and cell cycle control. TMPOβ/LAP2 plays a key role in nuclear envelope (NE) disassembly and reassembly during mitosis and linking chromatin to the NE in interphase. TMPOβ/LAP2 binds lamin B1 and chromosomes in a phosphorylation dependent manner. TMPOβ/LAP2, either together with germ-cell-less (GCL) or alone, can repress the transcriptional activity of the elongation factor 2-dimerization partner (E2F-DP) heterodimer. TMPOα/LAP2 has a role in regulating the dynamics of the nuclear lamina and this interaction is required for nuclear growth after mitosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB5700183	R1280	M9067
Sigma-Aldrich L3414 Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse	Sigma-Aldrich SAB5700183 Anti-TMPO antibody produced in rabbit	Sigma-Aldrich R1280 Monoclonal Anti-RPA/p34 antibody produced in mouse	Sigma-Aldrich M9067 Anti-MRP1 antibody,Mouse monoclonal
description ~2 mg/mL, clone 6E10, purified immunoglobulin, buffered aqueous solution	description -	description ~2 mg/mL, clone 9H8, purified immunoglobulin, buffered aqueous solution	description clone QCRL-1, purified from hybridoma cell culture
Gene Information human ... TMPO(7112)	Gene Information human ... TMPO(7112)	Gene Information human ... RPA2(6118)	Gene Information human ... ABCC1(4363)
antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture
biological source mouse	biological source rabbit	biological source mouse	biological source mouse
clone 6E10, monoclonal	clone polyclonal	clone 9H8, monoclonal	clone QCRL-1, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity human	species reactivity mouse, rat, human	species reactivity human, monkey	species reactivity human

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