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Anti-Leukemia Inhibitory Factor antibody produced in goat

Name: Anti-Leukemia Inhibitory Factor antibody produced in goat
Brand: SIGMA
Price: 669 USD
Availability: InStock

IgG fraction of antiserum, lyophilized powder

Synonym(s):

CDF, D factor, DIA, Emfilermin, LIF, differentiation inhibitory activity

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Size/SKU	Availability	Price
1 mg	Available to ship TODAYfromMILWAUKEE	$669.00

About This Item

UNSPSC Code:

51111800

NACRES:

NA.43

MDL number:

MFCD00283185

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, Neutral, WB

Citations:

$669.00

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Properties

biological source

goat

Quality Segment

100

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse

technique(s)

immunohistochemistry: suitable, neutralization: suitable, western blot: suitable

UniProt accession no.

P09056

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LIF(3976)
mouse ... Lif(16878)

Description

Immunogen

purified, E. coli-derived, recombinant mouse LIF.

Application

For Western blot a concentration of 0.1 μg/ml is recommended. For immunohistochemistry, a working dilution of 5-15 μg/ml is recommended. The neutralization dose is typically 80-480 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.

Optimal dilutions should be determined by the end user.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

The antibody neutralizes the biological activity of recombinant mouse LIF and also neutralizes the biologicl activity of recombinant human LIF at a 10-fold higher IgG concentration. Based on ELISA and immunoblotting, this antibody shows <10% cross-reactiity with recombinant human LIF.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Preparation Note

Protein A or G purified

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	L1044	L0669	HPA018844
$Anti-Leukemia Inhibitory Factor antibody produced in goat IgG fraction of antiserum, lyophilized powder$ Sigma-Aldrich L9152 Anti-Leukemia Inhibitory Factor antibody produced in goat	Sigma-Aldrich L1044 Anti-Leukemia Inhibitory Factor antibody produced in goat	Sigma-Aldrich L0669 Monoclonal Anti-Leukemia Inhibitory Factor antibody produced in mouse	Sigma-Aldrich HPA018844 Anti-LIF antibody produced in rabbit
description IgG fraction of antiserum, lyophilized powder	description affinity isolated antibody, lyophilized powder	description clone 9824, purified immunoglobulin, lyophilized powder	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source goat	biological source goat	biological source mouse	biological source rabbit
form lyophilized powder	form lyophilized powder	form lyophilized powder	form buffered aqueous glycerol solution
Gene Information human ... LIF(3976) mouse ... Lif(16878)	Gene Information mouse ... Lif(16878)	Gene Information human ... LIF(3976)	Gene Information human ... LIF(3976)

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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