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Anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr¹⁴⁷²) antibody produced in rabbit

Name: Anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr<SUP>1472</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 718 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-phospho-NMDA NR2B (pTyr¹⁴⁷²)

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$718.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD04040527

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ELISA (i), ICC, IP, WB

Citations:

$718.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 180 kDa

species reactivity

mouse, rat, Xenopus, chicken, human

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:1,000 using rat brain (hippocampal) homogenate

UniProt accession no.

Q13224

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr1472)

Gene Information

human ... GRIN2B(2904)
mouse ... Grin2b(14812)
rat ... Grin2b(24410)

Description

General description

Rabbit polyclonal anti-phospho- N-Methyl D-Aspartate NR2B Subunit (pTyr-1472) antibody specifically recognizes rat receptor NR2B subunit (~180 kDa) phosphorylated on tyrosine 1472. It does not crossreact with non-phosphorylated NMDA NR2B. It is likely to detect human, mouse, chicken and Xenopus NMDA NR2B based on sequence homology.

Specifically recognizes the NMDA receptor NR2B subunit phosphorylated on tyrosine 1472. Does not cross-react with non-phosphorylated NMDAR. Two additional lower molecular weight immunoreactive bands are present in immunoblots of rat brain.

The ionotropic glutamate receptor is a prominent ligand-gated and voltage-gated ion channel in excitatory synaptic transmission in the mammalian central nervous system. The glutamate receptor was named the NMDA receptor for its agonist, N-methyl-D-aspartate. The NMDA channel is regulated by its phosphorylation. Functional NMDA receptors contain an active invariant subunit (NMDAR1) associated with one of several variable regulatory subunits. NR2B is one of the variant regulatory subunits associated with the active subunit NMDAR1 and localized primarily to the hippocampus and forebrain. Tyrosine phosphorylation sites on NR2B have been identified. Phosphorylation of tyrosine 1472 on the NR2B subunit is largely dependent on Fyn, a member of the tyrosine kinase oncogene family which regulates long-term potentiation, spatial learning, and hippocampal development, and is elevated upon the long-term potentiation (LTP) induction of hippocampal CA1 neurons. Tyrosine 1472 phosphorylation of NR2B is important for synaptic plasticity.

Immunogen

synthetic phosphorylated peptide derived from the region of NMDA receptor NR2B that is phosphorylated on tyrosine 1472.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit polyclonal anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr-1472) antibody is used to tag the pTyr1472-NR2B subunit of NMDA for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of pTyr1472 phosphorylated NR2B subunit of NMDA in neural functions such as long-term potentiation (LTP) and synaptic plasticity.

Physical form

Solution in 10 mM HEPES, pH 7.5, and 150 mM NaCl containing 100 μg/ml BSA and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	M265	SAB4504677	SAB2104208
Sigma-Aldrich M2442 Anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr¹⁴⁷²) antibody produced in rabbit	Sigma-Aldrich M265 Anti-Glutamate Receptor NMDAR2B (NR2B) antibody produced in rabbit	Sigma-Aldrich SAB4504677 Anti-phospho-NMDAR2B (pTyr¹³³⁶) antibody produced in rabbit	Sigma-Aldrich SAB2104208 Anti-GRIN2B antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, lyophilized powder	description affinity isolated antibody	description affinity isolated antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
mol wt antigen 180 kDa	mol wt antigen 180 kDa	mol wt antigen 166 kDa	mol wt 163 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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