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Monoclonal Anti-Microphthalmia antibody produced in mouse

Name: Monoclonal Anti-Microphthalmia antibody produced in mouse
Brand: SIGMA
Price: 707 USD
Availability: InStock

clone C5, purified immunoglobulin

Synonym(s):

Anti-Mi

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100 μg	Check Cart for Availability	$707.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00802742

Conjugate:

unconjugated

Clone:

C5, monoclonal

Application:

IHC (p), IP, WB

Citations:

$707.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

C5, monoclonal

form

buffered aqueous solution

mol wt

antigen 52-56 kDa

species reactivity

mouse, rat, human

concentration

0.5-1.0 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable using 2 μg/mg protein lysate, western blot: 1:500 using 10 μg of Mouse brain lysates

isotype

IgG1

UniProt accession no.

O75030

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MITF(4286)
mouse ... Mitf(17342)
rat ... Mitf(25094)

Description

General description

Microphthalmia (Mi in mouse or MITF in human) is a basic helix-loop-helix-leucine zipper (BHLH–ZIP) transcription factor involved in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium, i.e. cells responsible for hair, skin, and eye color. It activates the expression of the melanocyte specific genes tyrosinase and TRP1 (tyrosinase-related protein 1) by binding as a homo- or heterodimer to a symmetrical DNA sequence (E box) (5′-CATGTG-3′) located within the M box found in their promoters. Microphthalmia also appears to be involved in the differentiation of mast cells, osteoclasts, basophils and natural killer cells.
Microphthalmia is expressed in a limited number of cell types including heart, mast cells, osteoclast precursors, and melanocytes. There are a number of different isoforms of microphthalmia resulting from alternative splicing and alternative promotors. These isoforms differ at their amino-termini and in their expression patterns.

Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.

Immunogen

N-terminal fragment of human microphthalmia protein.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal clone C5 anti-Microphthalmia antibody is used to tag serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia for detection and quantitation by techniques such as immunoblotting (doublet of 52-56 kDa), immunoprecipitation, immunohistochemistry on formalin-fixed paraffin embedded or frozen tissue sections, and gel shift. It is used as a probe to determine the roles of microphthalmia in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	MAB3747	HPA003259	SAB5700722
Sigma-Aldrich M6065 Monoclonal Anti-Microphthalmia antibody produced in mouse	Sigma-Aldrich MAB3747 Anti-Microphthalmia (Mi) Antibody, clone C5	Sigma-Aldrich HPA003259 Anti-MITF antibody produced in rabbit	Sigma-Aldrich SAB5700722 Anti-MITF antibody produced in rabbit
description clone C5, purified immunoglobulin	description clone C5, Chemicon^®, from mouse	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution	description -
species reactivity mouse, rat, human	species reactivity mouse	species reactivity human	species reactivity mouse, human
conjugate unconjugated	conjugate -	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
Gene Information human ... MITF(4286) mouse ... Mitf(17342) rat ... Mitf(25094)	Gene Information -	Gene Information human ... MITF(4286)	Gene Information human ... MITF(4286)
clone C5, monoclonal	clone C5, monoclonal	clone polyclonal	clone polyclonal
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody

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