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Anti-Mitofusin-2 (N-Terminal) antibody produced in rabbit

Name: Anti-Mitofusin-2 (N-Terminal) antibody produced in rabbit
Brand: SIGMA
Price: 549.95 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Mitofusin 2 Antibody, Mitofusin 2 Antibody - Anti-Mitofusin-2 (N-Terminal) antibody produced in rabbit, Anti-CMT2A, Anti-CMT2A2, Anti-CPRP1, Anti-KIAA0214, Anti-MARF, Anti-Mfn2

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Size/SKU	Availability	Price
200 μL	Estimated to ship TODAYfromMILWAUKEE	$647.00 $549.95

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03455396

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB CL

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~86 kDa

species reactivity

mouse, human, rat

technique(s)

immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate, indirect immunofluorescence: 20-30 μg/mL using differentiated mouse C2 cells, western blot (chemiluminescent): 0.5-1 μg/mL using extracts of rat or mouse brain mitochondria

UniProt accession no.

O95140

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MFN2(9927)
mouse ... Mfn2(170731)
rat ... Mfn2(64476)

Description

General description

Mitofusins (Mfn1 and Mfn2) are the mammalian homologs of the Drosophila protein fuzzy onion (Fzo). They are transmembrane GTPases embedded in the outer membrane of mitochondria, essential for fusion of mitochondria in mammalian cells. Mfn1 and Mfn2 form homotypic and heterotypic complexes that are functional for fusion. Mitochondrial fusion is also important for cell growth, mitochondrial membrane potential, respiration, and embryonic development. Mice deficient in either Mfn1 or Mfn2 die in mid-gestation. Mfn2 mutant embryos have a specific and severe disruption of a layer of the placenta. Mitofusin 2 is broadly expressed, with highest expression in heart and skeletal muscle and is induced during myogenesis. Repression of Mfn2 causes morphological and functional fragmentation of the mitochondrial network into independent clusters and reduces mitochondrial membrane potential and glucose oxidation. Thus, Mfn2 is essential for the maintenance of mitochondrial network and controls mitochondrial metabolism. This Mfn2-dependent regulatory mechanism is disturbed in obesity by reduced Mfn2 expression. Mutations in Mitofusin 2 cause Charcot-Marie-Tooth neuropathy type 2A, a neurological disorder that results from degeneration of axons in peripheral nerves.

Immunogen

synthetic peptide corresponding to amino acid residues 38-55 of human mitofusin 2 with C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in both rat and mouse mitofusin 2.

Application

Anti-Mitofusin 2 (N-terminal) antibody is suitable for immunoblotting (~86 kDa), immunoprecipitation, and immunofluorescence applications.
By immunoblotting, a working antibody concentration of 0.5-1 mg/mL is recommended using an extracts of rat and mouse brain mitochondria and a chemiluminescent detection reagent.
By indirect immunofluorescence, a working antibody concentration of 20-30 mg/mL is recommended using differentiated mouse C2 cells.
5-10 mg of the antibody immunoprecipitates mitofusin 2 from HeLa human epithelioid carcinoma cell lysate.

Anti-mitofusion 2 antibody may be used for immunoprecipitation in HeLa cells; immunoblotting in mouse and rat brain mitochondia and immunoflurescence in mouse C2 cells

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Anti-Mitofusin 2 (N-terminal) antibody recognizes human, rat, and mouse mitofusin 2. Detection of the mitofusin 2 band by immunoblotting is specifically inhibited with the immunizing peptide.

The antibody is specific for N-terminal of mitofusin 2 (~86 kDa)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB5201402	SAB5201405	SAB5201407
Sigma-Aldrich M6319 Anti-Mitofusin-2 (N-Terminal) antibody produced in rabbit	Sigma-Aldrich SAB5201402 Monoclonal Anti-Mitofusin - Apc antibody produced in mouse	Sigma-Aldrich SAB5201405 Monoclonal Anti-Mitofusin - Hrp antibody produced in mouse	Sigma-Aldrich SAB5201407 Monoclonal Anti-Mitofusin - Percp antibody produced in mouse
description affinity isolated antibody, buffered aqueous solution	description clone S153-5, purified immunoglobulin	description clone S153-5, purified immunoglobulin	description clone S153-5, purified immunoglobulin
conjugate unconjugated	conjugate Allophycocyanin conjugate	conjugate peroxidase conjugate	conjugate Peridinin-Chlorophyll-Protein Complex
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
biological source rabbit	biological source mouse	biological source mouse	biological source mouse
antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
shipped in dry ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
Gene Information human ... MFN2(9927) mouse ... Mfn2(170731) rat ... Mfn2(64476)	Gene Information mouse ... Mfn2(170731)	Gene Information mouse ... Mfn2(170731)	Gene Information mouse ... Mfn2(170731)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c