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Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse

Name: Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse
Brand: SIGMA
Price: 839 USD
Availability: InStock

clone 1B3B9, purified immunoglobulin, buffered aqueous solution

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Size/SKU	Availability	Price
200 μg	Check Cart for Availability	$839.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD01633614

Conjugate:

unconjugated

Clone:

1B3B9, monoclonal

Application:

ARR, IP, WB

Citations:

$839.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B3B9, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

human, rat, mouse

technique(s)

immunoprecipitation (IP): 4 μg using denatured ERK-2 from a mouse 3T3 fibroblast lysate, microarray: suitable, western blot: 0.5 μg/mL using lysates from human A431 cells, mouse 3T3 fibroblasts or rat L6 cells

isotype

IgG2a

UniProt accession no.

P28482

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)

Description

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Anti-MAP Kinase 2 recognizes the 42 kDa MAPK2 encoded by the mapk gene. The antibody may weakly react with MAPK1 (ERK1) and only binds denatured MAP kinase.

Immunogen

recombinant mouse MAP kinase.

Application

Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody is suitable for detection by immunoblotting, at a working concentration of 0.5 μg/mL, using whole cell lysates of human A431, mouse 3T3 fibroblasts or rat L6 cells. Detection of MAPK2 (ERK-2) was possible in human colon adenocarcinoma cells, HT-29. Immunoprecipitation applications in mouse 3T3 fibroblasts require a concentration of 4 μg. The antibody may be suitable for protein microarray.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M5670	M3807	M8159
Sigma-Aldrich M7431 Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse	Sigma-Aldrich M5670 Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit	Sigma-Aldrich M3807 Anti-MAP Kinase, Non-Phosphorylated ERK antibody, Mouse monoclonal	Sigma-Aldrich M8159 Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse
description clone 1B3B9, purified immunoglobulin, buffered aqueous solution	description whole antiserum	description clone ERK-NP2, purified from hybridoma cell culture	description clone MAPK-YT, ascites fluid
clone 1B3B9, monoclonal	clone polyclonal	clone ERK-NP2, monoclonal	clone MAPK-YT, monoclonal
Gene Information human ... MAPK1(5594) mouse ... Mapk1(26413) rat ... Mapk1(116590)	Gene Information human ... MAPK1(5594), MAPK3(5595) mouse ... Mapk1(26413), Mapk3(26417) rat ... Mapk1(116590), Mapk3(50689)	Gene Information human ... MAPK1(5594), MAPK3(5595) rat ... Mapk1(116590), Mapk3(50689)	Gene Information human ... MAPK1(5594), MAPK3(5595) mouse ... Mapk1(26413), Mapk3(26417) rat ... Mapk1(116590), Mapk3(50689)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form whole antiserum	antibody form purified from hybridoma cell culture	antibody form ascites fluid
species reactivity human, rat, mouse	species reactivity human, rat, mouse	species reactivity human, rat	species reactivity human, Caenorhabditis elegans, Xenopus, Drosophila, hamster, rat, bovine, mouse, yeast
biological source mouse	biological source rabbit	biological source mouse	biological source mouse

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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