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Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr²²³) antibody produced in rabbit

Name: Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr<SUP>223</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 658 USD
Availability: InStock

affinity isolated antibody, buffered aqueous glycerol solution

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0.1 mL	Check Cart for Availability	$658.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD02097162

Conjugate:

unconjugated

Clone:

polyclonal

Application:

WB CL

Citations:

$658.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human, mouse, rat

technique(s)

western blot (chemiluminescent): 1:2,000 using sodium chloride and vanadate-treated 293 cells

UniProt accession no.

P45985

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr223)

Gene Information

human ... MAP2K4(6416)
mouse ... Map2k4(26398)

Description

General description

SAPK/ERK-1 kinase (SEK1) also called MKK4 or Jun kinase kinase (JNKK) activates the stress activated protein kinase (SAPK). There are other mechanisms by which SAPK may be activated but SEK1 being the immediate upstream activator defines the ensuing protein kinase cascade by the c-Jun transcription factor. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at position 219 and 223 respectively by MEKK. High levels of JNK/SAPK activity are reported to induce apoptosis by the activation of c-Jun. Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK1, JNKK1 (phosphothreonine 223) detects SEK1/MKK4 protein when activated by phosphorylation at Thr223.

Immunogen

synthetic phospho-Thr²²³ peptide corresponding to residues around Thr²²³ of human SEK1.

Application

Anti-phospho-MAP Kinase Kinase 4 may be used for immunoblotting at a working dilution of 1:2,000 using sodium chloride and vanadate-treated 293 cells.

Biochem/physiol Actions

Does not react with non-phosphorylated MKK4/SEK1 or phosphorylated MKK3, MEK-1 or MEK-2.

Physical form

Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M7683	SAB5700307	SAB5700309
Sigma-Aldrich M7433 Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr²²³) antibody produced in rabbit	Sigma-Aldrich M7683 Anti-phospho-MAP Kinase Kinase 1/2 (pSer^217/221) antibody produced in rabbit	Sigma-Aldrich SAB5700307 Anti-Phospho-MAP3K5-S1033 antibody produced in rabbit	Sigma-Aldrich SAB5700309 Anti-Phospho-MAP3K5-T918 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution	description -	description -
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate -
Gene Information human ... MAP2K4(6416) mouse ... Map2k4(26398)	Gene Information human ... MAPK1(5594) mouse ... Mapk1(26413) rat ... Mapk1(116590)	Gene Information human ... MAP3K5(4217)	Gene Information human ... MAP3K5(4217)
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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