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Anti-MAPK3/ERK1 Antibody

Name: Anti-MAPK3/ERK1 Antibody
Brand: SIGMA
Price: 540 USD
Availability: InStock

rabbit polyclonal

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$540.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD01633615

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IP, WB

Citations:

$540.00

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Properties

Product Name

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen, ERK-1 44 kDa, antigen, ERK-2 42 kDa

species reactivity

mouse, rat, human

technique(s)

immunoprecipitation (IP): 5 μg using NIH 3T3 mouse fibroblast lysates, microarray: suitable, western blot: 1:5,000 using NIH 3T3 mouse fibroblast lysate or rat brain extract

UniProt accession no.

P27361

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPK3(5595)
mouse ... Mapk3(26417)
rat ... Mapk3(50689)

Description

General description

Mitogen-activated protein kinase 3 (ERK1) is mapped to human chromosome 16p11.2. It has a serine/threonine domain and a transactivation domain (TAD) and a nuclear localization sequence (NLS).

Immunogen

synthetic peptide corresponding to amino acids 351-368 of human ERK-1.

Application

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit has been used in:

immunofluorescence
immunoprecipitation
western blotting.

Biochem/physiol Actions

By immunoblotting, the antibody reacts with ERK-1 and ERK-2.

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), c-Jun N-terminal kinases (JNK) and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	E1523	M3807	M7431
$Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich M7927 Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit	$Anti-ERK5 (big-MAPK, BMK1) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich E1523 Anti-ERK5 (big-MAPK, BMK1) antibody produced in rabbit	Sigma-Aldrich M3807 Anti-MAP Kinase, Non-Phosphorylated ERK antibody, Mouse monoclonal	Sigma-Aldrich M7431 Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse
description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description clone ERK-NP2, purified from hybridoma cell culture	description clone 1B3B9, purified immunoglobulin, buffered aqueous solution
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
clone polyclonal	clone polyclonal	clone ERK-NP2, monoclonal	clone 1B3B9, monoclonal
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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