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Anti-AtMPK3 antibody produced in rabbit

Name: Anti-AtMPK3 antibody produced in rabbit
Brand: SIGMA
Price: 710 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

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Synonym(s):

Anti-Arabidopsis thaliana MAP Kinase 3

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Size/SKU	Availability	Price
200 μL	Estimated to ship onSeptember 21, 2026fromMILWAUKEE	$710.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD07370259

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 43 kDa

species reactivity

Arabidopsis thaliana

technique(s)

western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves, western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Arabidopsis thaliana ... ATMPK3(823706)

Description

General description

The protein kinase AtMPK3 is a component of the MAP kinase cascade.

Immunogen

Synthetic peptide corresponding to amino acids at the C-terminus of Arabidopsis thaliana MPK3. This sequence is specific to AtMPK3 (not found in the other 20 known AtMPK isoforms). It has limited homology (58% identity) to the AtMPK6 isoform and shares significant homology (67% identity, continuous epitope) with the Nicotiana tabacum ortholog WIPK.

Application

Anti-AtMPK3 antibody produced in rabbit has been used in immunoblotting analysis.

Biochem/physiol Actions

Anti-AtMPK3 recognizes AtMPK3.

AtMPK3 is a component of the plant MAPK signaling cascade that functions downstream of the FLS2 receptor and regulates the innate response to bacterial and fungal infections. AtMPK3 is also involved in the phosphorylation of VIP1 in response to Agrobacterium infection and the subsequent transfer of the bacterial T-DNA into the nucleus. RNAi mediated inhibition of AtMPK6 causes prolonged activation of AtMPK3 . Anti-AtMPK3 antibody is specific for AtMPK3 (43 kDa). The product also reacts with Nicotiana tabacum and p56 in Papaver rhoeas . In immunoblotting, staining of AtMPK3 is specifically inhibited with AtMPK3 immunizing peptide (Arabidopsis thaliana, amino acids 359-370).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A6979	N6537
Sigma-Aldrich M8318 Anti-AtMPK3 antibody produced in rabbit	Sigma-Aldrich A6979 Anti-AtMPK4 antibody produced in rabbit	Sigma-Aldrich N6537 Anti-NPTII antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, liquid	description affinity isolated antibody, buffered aqueous solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source rabbit	biological source rabbit
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
technique(s) western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves, western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves	technique(s) western blot: 0.5-1 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana and Nicotiana tabacum leaves	technique(s) western blot: 2-4 μg/mL using extract (cytosolic fraction) from the leaves of transgenic Nicotiana tabacum expressing E. coli NPTII

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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