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M9532

Mannitol Dehydrogenase from Leuconostoc mesenteroides

lyophilized powder, ≥60 units/mg protein

Synonym(s):

polyol dehydrogenases, Mannitol:NAD oxidoreductase

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Size/SKUAvailabilityPrice
5 units
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$241.00
50 units
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$1,110.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥60 units/mg protein

$241.00


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form

lyophilized powder

Quality Segment

specific activity

≥60 units/mg protein

mol wt

136 kDa

storage temp.

−20°C

General description

The molecular weight of mannitol dehydrogenase is 136 kDa.
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.

Application

It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
Can be used for the production of D-mannitol.
This preparation is useful in the determination of mannitol in urine.

Biochem/physiol Actions

Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.

Physical form

Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol

Preparation Note

Partially purified

Analysis Note

Assayed according to the method of Yamanaka, Meth. Enzymol., 41(B), 138 (1975).

Other Notes

One unit will reduce 1.0 μmole of D-fructose per min in the presence of NADH at pH 5.3 at 30°C. One unit of enzyme, as defined above, is equivalent to approximately 1.8 units of enzyme, as described by Lunn et al., one unit will oxidize 1.0 μmole of D-mannitol per min in the presence of NAD at pH 8.6 at 40°C.

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346774L5135G5885
description

lyophilized powder, ≥60 units/mg protein

description

Catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate.

description

lyophilized powder, ≥60 units/mg protein

description

lyophilized powder, >= 550 units/mg protein (biuret)

specific activity

≥60 units/mg protein

specific activity

≥200 units/mg protein

specific activity

≥60 units/mg protein

specific activity

>= 550 units/mg protein (biuret)

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

mol wt

136 kDa

mol wt

-

mol wt

245 kDa

mol wt

128 kDa

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

300


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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