Yes, this antibody can be used with formalin-fixed, paraffin-embedded brain tissues.
N2280
Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse
clone NOS-B1, ascites fluid
Synonym(s):
Anti-bNOS
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| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Available to ship TODAYfromMILWAUKEE | $568.00 |
0.2 mL | Available to ship TODAYfromMILWAUKEE | $727.00 |
About This Item
Conjugate:
unconjugated
Clone:
NOS-B1, monoclonal
Application:
ARR, WB
Citations:
61
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NOS-B1, monoclonal
mol wt
antigen 150-160 kDa
contains
15 mM sodium azide
species reactivity
rat, goat, pig, human
technique(s)
microarray: suitable, western blot: 1:3,000 using fresh rat cerebellum extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NOS1(4842)
rat ... Nos1(24598)
General description
Monoclonal Anti-Nitric Oxide Synthase-Brain (bNOS) (mouse IgG1 isotype) is derived from the NOS-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Nitric oxide synthase (NOS) has been localized in many different cell types. Type I NOS is found in neurons. It is a 150-160 kDa protein, also called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+- regulated NOS (cNOS). bNOS is present also in skeletal muscle, where it is complexed with dystrophin, and is absent in Duchenne′s muscular dystrophy, which perhaps accounts for symptoms of the disease.
Immunogen
recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.
Application
Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse has been used in:
- western blot analysis
- immunocytochemistry
- double immunofluorescence
- immunofluorescence staining
- enzyme-linked immunosorbent assay (ELISA)
- dot blot immunoassay
- immunohistochemical staining
Biochem/physiol Actions
Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody is specific for nitric oxide synthase (NOS) derived from brain (bNOS, 150-160 kDa and several breakdown products of lower M.W.). The product does not bind to NOS derived from macrophages (mNOS) and endothelial cells (eNOS). This antibody is specific for bNOS in humans, goats, pigs and rats.
Nitric oxide synthase is an enzyme that catalyzes the formation of nitric oxide. Nitric oxide is responsible for regulating several biochemical functions such as hemostasis, neurotransmission and vascular injury response . Mutations in nitric oxide synthase gene have been associated with diabetic nephropathy.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone NOS-B1, ascites fluid | description IgG fraction of antiserum, buffered aqueous solution | description clone NOS-3F7-B11-B5, ascites fluid | description clone NOS-IN, ascites fluid |
| biological source mouse | biological source rabbit | biological source mouse | biological source mouse |
| species reactivity rat, goat, pig, human | species reactivity human, rat | species reactivity mouse, bovine, rat | species reactivity mouse, rat |
| antibody form ascites fluid | antibody form IgG fraction of antiserum | antibody form ascites fluid | antibody form ascites fluid |
| clone NOS-B1, monoclonal | clone polyclonal | clone NOS-3F7-B11-B5, monoclonal | clone NOS-IN, monoclonal |
| Gene Information human ... NOS1(4842) | Gene Information human ... NOS1(4842) | Gene Information mouse ... Nos1(18125) | Gene Information human ... NOS2(4843) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
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Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Data sheet states this antibody can be used in ffpe tissue and paraformaldehyde perfusion-fixed brain tissue. Can this be used in ffpe brain tissue?
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