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Anti-Nucleolin antibody produced in rabbit

Name: Anti-Nucleolin antibody produced in rabbit
Brand: SIGMA
Price: 196 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-C23

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$196.00
200 μL	Available to ship TODAYfromMILWAUKEE	$665.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02686475

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

$196.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 105 kDa

species reactivity

mouse, rat, human

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): 1-2 μg using 250 μg RIPA lysate of human HeLa cells, indirect immunofluorescence: 10-20 μg/mL using human HeLa cells, western blot: 0.5-1.0 μg/mL using whole extract of mouse NIH3T3 cells or rat PC12 cells

UniProt accession no.

P19338

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NCL(4691)
mouse ... Ncl(17975)
rat ... Ncl(25135)

Description

General description

Anti-Nucleolin is produced in rabbit using as immunogen a synthetic peptide of human nucleolin with C-terminal added cysteine conjugated to KLH. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. The N-terminal domain comprises of highly acidic regions separated by basic sequences and contains multiple phosphorylation sites. The central domain contains four RNA-binding domains (RBD or RRM) and the C-terminal domain (GAR or RGG) is rich in glycine, arginine, and phenylalanine residues. N and C terminal domains are involved in protein-protein interaction whereas the central domain is involved in specific interactions with nucleic acids. Nucleolin is mainly localized in the nucleus and at the cell surface of cultured tumor cells and endothelial cells of angiogenic vessels in vivo.

Nucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. It has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and nucleo-cytoplasmic transport.

Rabbit polyclonal anti-Nucleolin antibody recognizes human, mouse, and rat nucleolin. Detection of the nucleolin band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acid residues 2-17 of human nucleolin with C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by 4 amino acids in rat and mouse nucleolin and by 1 amino acid in the rat homolog protein NRP (nucleolin related protein).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit polyclonal anti-Nucleolin antibody is used to tag nucleolin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting (105 kDa), immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of nucleolin in ribosome maturation, cytokinesis, nucleogenesis, cell proliferation and growth, and nucleo-cytoplasmic transport in exponentially growing eukaryotic cells.

Biochem/physiol Actions

Nucleolin also exhibits auto degradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2 and protein kinase C. Nucleolin might function as a cell surface receptor for lipoproteins, viruses, extracellular matrix, growth factors and other molecules. It interacts with telomerase, which is critical for the nucleolar localization of telomerase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	ZRB1415	SAB1305550	HPA023981
Sigma-Aldrich N2662 Anti-Nucleolin antibody produced in rabbit	Sigma-Aldrich ZRB1415 Anti-Nucleolin Antibody, clone 5D13 ZooMAb^® Rabbit Monoclonal	$MONOCLONAL ANTI-NUCL antibody produced in mouse clone 130CT43.8.5, IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich SAB1305550 MONOCLONAL ANTI-NUCL antibody produced in mouse	Sigma-Aldrich HPA023981 Anti-NCL antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description recombinant, expressed in HEK 293 cells	description clone 130CT43.8.5, IgG fraction of antiserum, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form purified antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
technique(s) indirect immunofluorescence: 10-20 μg/mL using human HeLa cells, western blot: 0.5-1.0 μg/mL using whole extract of mouse NIH3T3 cells or rat PC12 cells, immunoprecipitation (IP): 1-2 μg using 250 μg RIPA lysate of human HeLa cells	technique(s) western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunocytochemistry: suitable	technique(s) immunofluorescence: 1:10-1:50, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000	technique(s) immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200
shipped in dry ice	shipped in ambient	shipped in wet ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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