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Anti-NPTII antibody produced in rabbit

Name: Anti-NPTII antibody produced in rabbit
Brand: SIGMA
Price: 639 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-Neomycin Phosphotransferase II

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$639.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01633903

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

$639.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 30 kDa

species reactivity

plant

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

western blot: 2-4 μg/mL using extract (cytosolic fraction) from the leaves of transgenic Nicotiana tabacum expressing E. coli NPTII

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the E. coli transposon Tn5.

Immunogen

synthetic peptide corresponding to amino acids 133-150 located at the mid-region of E. coli NPTII, conjugated to KLH.

Application

Anti-NPTII antibody has been used in immunoblotting.

Biochem/physiol Actions

The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.

The antibody recognizes bacterial NPTII expressed in transgenic tobacco plants.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	HPA051497	HPA077062	SAB2107393
Sigma-Aldrich N6537 Anti-NPTII antibody produced in rabbit	Sigma-Aldrich HPA051497 Anti-NPTN antibody produced in rabbit	Sigma-Aldrich HPA077062 Anti-NPTX1	Sigma-Aldrich SAB2107393 Anti-NPTX1 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody	description affinity isolated antibody
Quality Level 200	Quality Level 100	Quality Level -	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
shipped in dry ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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