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Anti-Notch1 antibody, Mouse monoclonal

Name: Anti-Notch1 antibody, Mouse monoclonal
Brand: SIGMA
Price: 813 USD
Availability: InStock

clone mN1A, purified from hybridoma cell culture

Synonym(s):

Notch antibody

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$813.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03455659

Conjugate:

unconjugated

Clone:

mN1A, monoclonal

Application:

ARR, ELISA (i), ICC, WB

Citations:

$813.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

mN1A, monoclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using extracts of cells stably transfected with a truncated Notch1 (32D-mN1-ICDOP that expresses the intracellular mouse Notch1 molecule-65 kDa).

isotype

IgG1

UniProt accession no.

P46531

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOTCH1(4851)
mouse ... Notch1(18128)

Description

General description

Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.

The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems.In mammals, four Notch genes were identified (Notch1-4) in a wide variety of cells and play a crucial role in differentiation and development. Notch proteins contain three main domains: an extracellular domain containing tandem epidermal growth factor repeats, and three lin-12/Notch repeats, a transmembrane domain, and an intracellular domainthat contains six cdc1-/SW16/ankyrin repeats, nuclear localization signals, and a C-terminal OPA/PEST region.

Immunogen

recombinant protein containing the cdc10-NCR region of mouse Notch1.

Application

Anti-Notch1 antibody, mouse monoclonal has been used in:

enzyme linked immunosorbent assay (ELISA)
immunoblotting
immunocytochemistry
immunohistochemistry

Biochem/physiol Actions

NOTCH is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition.Fibronectin is suggested to enhance cell adhesion, spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin.

Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB2101618	SAB1402292	SAB4700742
Sigma-Aldrich N6786 Anti-Notch1 antibody, Mouse monoclonal	Sigma-Aldrich SAB2101618 Anti-NOTCH1 antibody produced in rabbit	Sigma-Aldrich SAB1402292 Monoclonal Anti-NOTCH1 antibody produced in mouse	Sigma-Aldrich SAB4700742 Monoclonal Anti-Notch 1 antibody produced in mouse
description clone mN1A, purified from hybridoma cell culture	description affinity isolated antibody	description clone 4G1, purified immunoglobulin, buffered aqueous solution	description clone mN1A, purified immunoglobulin, buffered aqueous solution
species reactivity mouse, human	species reactivity human	species reactivity mouse, human	species reactivity human, mouse
antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form purified immunoglobulin
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source rabbit	biological source mouse	biological source mouse
clone mN1A, monoclonal	clone polyclonal	clone 4G1, monoclonal	clone mN1A, monoclonal
Gene Information human ... NOTCH1(4851) mouse ... Notch1(18128)	Gene Information human ... NOTCH1(4851)	Gene Information human ... NOTCH1(4851)	Gene Information human ... NOTCH1(4851)

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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