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Anti-p62/SQSTM1 (C-terminal) antibody produced in rabbit

Name: Anti-p62/SQSTM1 <B>(</B>C-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 601 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Sequestosome 1, Anti-Ubiquitin-binding protein p62

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$580.00 $601.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

$601.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~62 kDa

species reactivity

rat, mouse, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2-4 μg using whole extract of NIH-3T3 cells, western blot: 2-4 μg/mL using whole extracts of rat PC12 cells, western blot: 4-8 μg/mL using whole extracts of human A549 cells.

UniProt accession no.

Q13501

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SQSTM1(8878)
mouse ... Sqstm1(18412)
rat ... Sqstm1(113894)

Description

General description

The p62 protein/sequestosome 1 (SQSTM1) comprises a Phox and Bem1p (PB1) domain at the NH2-terminal, a ZZ type zinc finger domain, a Pro, Glu, Ser, and Thr (PEST) region consisting of putative phosphorylation sites, and a ubiquitin-associated (UBA) domain at the COOH-terminal. The SQSTM1 gene is mapped to the human chromosome location 5q35.3.

Application

Rabbit polyclonal anti-SQSTM1 antibody has been used:

in immunoblotting
in immunoprecipitation
as a probe to determine the presence and roles of p62 protein/sequestosome 1 in processes such as autophagy

Biochem/physiol Actions

Anti-p62/SQSTM1 (C-terminal) recognizes human, rat, and mouse p62/SQSTM1.

The p62 protein/sequestosome 1 (SQSTM1) is a multifunctional protein, which binds to ubiquitin. It acts as a scaffold protein for the nuclear factor kappa-B (NF-ΚB) signaling pathway. SQSTM1 also regulates apoptosis and autophagy. Mutations in this gene lead to sporadic and familial Paget disease of bone. It is also associated with protein aggregation diseases, such as Lewy bodies in Parkinson′s disease, neurofibrillary tangles in Alzheimer′s disease, and huntingtin aggregates. SQSTM1 is commonly found in inclusion bodies containing polyubiquitinated protein aggregates that accumulate in several degenerative diseases. p62 polymerizes through its N-terminal Phox and Bem1p (PB1) domain and interacts with polyubiquitinated proteins through its C-terminal ubiquitin-associated (UBA) domain. p62 acts as a linker between protein aggregates and the autophagy machinery.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB5700845	HPA064165	MABN130
Sigma-Aldrich P0068 Anti-p62/SQSTM1 (C-terminal) antibody produced in rabbit	Sigma-Aldrich SAB5700845 Anti-SQSTM1/p62 antibody produced in rabbit	Sigma-Aldrich HPA064165 Anti-SQSTM1 antibody produced in rabbit	Sigma-Aldrich MABN130 Anti-SQSTM1 Antibody, clone 20F1.1
description affinity isolated antibody, buffered aqueous solution	description -	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody	description clone 20F1.1, from mouse
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source mouse
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
shipped in dry ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
technique(s) western blot: 4-8 μg/mL using whole extracts of human A549 cells., immunoprecipitation (IP): 2-4 μg using whole extract of NIH-3T3 cells, western blot: 2-4 μg/mL using whole extracts of rat PC12 cells	technique(s) immunoprecipitation (IP): 1:50-1:100, western blot: 1:500-1:2000, immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200	technique(s) immunofluorescence: 0.25-2 μg/mL, immunoblotting: 0.04-0.4 μg/mL	technique(s) immunohistochemistry: suitable, western blot: suitable

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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