This product is tested for use in western blot and has been used successfully in immunocytochemistry. Cells were stained with this antibody (1:200), followed by Goat Anti-Rabbit-AlexaFluor-555 secondary antibody. For details, please refer to the photos in the upper left of this page. The suitability for use in FACS would have to be determined experimentally by the end user.
P0999
Anti-Potassium Channel Kv10.1 (EAG-1) antibody produced in rabbit
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-Ether-a-go-go K+ channel 1, Anti-KCNH1
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| Size/SKU | Availability | Price |
|---|---|---|
50 μL | Check Cart for Availability | $356.00 |
200 μL | Check Cart for Availability | $974.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
3
$356.00
Check Cart for Availability
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human, mouse, rat
technique(s)
western blot: 1:200 using rat brain lysate and transfected cells HEK-KV10.1.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... KCNH1(3756)
mouse ... Kcnh1(16510)
rat ... Kcnh1(65198)
General description
Human genome codes for 40 voltage-gated K+ channels (KV). Voltage-gated-like ion channels (VGICs) are the third largest group of signaling molecules found in humans. K+ channels can be divided into four structural types based on their number of transmembrane regions and mode of activation, namely Kir- the inwardly rectifying 2-transmembrane K+ channels, K2P- 2-pore 4-transmembrane K+ channels, KCa- Ca2+-activated 6-transmembrane or 7-transmembrane K+ channels, and KV- voltage-gated 6-transmembrane K+ channels. KCNH1 (potassium voltage-gated channel H1) is a KV channel and is also known as KV10.1. Like all KV members, it is composed of four α-subunits with each containing six transmembrane α-helical segments, and a membrane-re-entering P-loop. These surround a central pore as homotetramers or heterotetramers. KCNH1 mRNA and protein are both abundantly found in the brain, though its peripheral tissue expression is restricted. This gene is localized to human chromosome 1q32.1.
Potassium Channel Kv10.1 (EAG-1) encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming α-subunit of a voltage-gated non-inactivating delayed rectifier potassium channel.
Immunogen
peptide GDPAKRKGWARFKDAC corresponding to residues 802-817 of rat KV10.1. This sequence is identical in mouse and has 14/16 residues identical in human.
Biochem/physiol Actions
KCNH1 (potassium voltage-gated channel H1) initiates a slowly activating, non-inactivating K+ current in a heterologous system. In skeletal muscles, it is expressed only during the phase when myoblasts exit the cell cycle and fuse, and thus, it plays an essential role in skeletal muscle development. This protein is found to be expressed in ∼70% human cancers, including gastric, mammary tumors, colon carcinoma, and sarcomas. This channel is implicated in neuronal disorders such as Zimmermann-Laband and Temple-Baraitser syndromes.
Specific for KV10.1 and does not cross react with KV10.2.
Physical form
Lyophilized from phosphate buffered saline containing 1% bovine serum albumin and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description affinity isolated antibody, lyophilized powder | description affinity isolated antibody, lyophilized powder | description affinity isolated antibody, lyophilized powder | description Chemicon®, from rabbit |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity purified immunoglobulin |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| Gene Information human ... KCNH1(3756) | Gene Information human ... KCNH2(3757) | Gene Information human ... KCNQ1(3784) | Gene Information rat ... Kcnh1(65198) |
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Storage Class
13 - Non Combustible Solids
WGK
WGK 2
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この抗体抗カリウムチャネルKv10.1(EAG-1)抗体 はFACSで二次抗体を使ってヒトの細胞表面を染めることができますか？
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