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P1782

Protein kinase Cα isozyme human

≥70% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

Synonym(s):

PKCα

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5 μg
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$542.00
20 μg
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$1,280.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Specific activity:
≥250 units/μg protein
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected insect cells

$542.00


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recombinant

expressed in baculovirus infected insect cells

Quality Segment

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥250 units/μg protein

mol wt

80-81 kDa by SDS-PAGE

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKCA(5578)

Biochem/physiol Actions

PKCα enhances the proliferation of several tumor cell lines in vitro and in vivo. Increases permeability of endothelial and epithelial tight junctions.
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Physical form

Supplied as a buffered aqueous glycerol solution, containing Trizma buffer, NaCl, DTT and Glutathione.

Other Notes

One unit will transfer 1 pmol of phosphate from ATP to Histone H1 in 1 minute at pH 7.4 at 30 °C

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This Item
P3287P0194SRP0263
description

≥70% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

description

>85% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

description

≥75% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous solution

description

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

specific activity

≥250 units/μg protein

specific activity

≥500 unit/mg solid

specific activity

-

specific activity

≥10000 units/μg

assay

≥70% (SDS-PAGE)

assay

>85% (SDS-PAGE)

assay

≥75% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

Gene Information

human ... PRKCA(5578)

Gene Information

human ... PRKCB(5579)

Gene Information

human ... PRKCZ(5590)

Gene Information

human ... PDE4B(24626)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

aqueous solution

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

mol wt

80-81 kDa by SDS-PAGE

mol wt

calculated mol wt 76.9 kDa, 80 kDa by SDS-PAGE

mol wt

76-80 kDa by SDS-PAGE

mol wt

109 kDa


Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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