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Monoclonal Anti-phospho-CaM Kinase IIα (pThr²⁸⁶) antibody produced in mouse

Name: Monoclonal Anti-phospho-CaM Kinase IIα (pThr<SUP>286</SUP>) antibody produced in mouse
Brand: SIGMA
Price: 768 USD
Availability: InStock

clone 22B1, purified immunoglobulin, buffered aqueous solution

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100 μL	Check Cart for Availability	$768.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD01322970

Conjugate:

unconjugated

Clone:

22B1, monoclonal

Application:

IF, WB

Citations:

$768.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

22B1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, mouse

technique(s)

indirect immunofluorescence: 1:100 using rat hippocampus (cold methanol fixation), western blot: 1:2,000

isotype

IgG1

UniProt accession no.

P11798

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr286)

Gene Information

mouse ... Camk2a(12322)
rat ... Camk2a(25400)

Description

General description

Intracellular Calcium (Ca²⁺) acts as major secondary messenger that mediates signalling induced by hormones and neurotransmitters. Cellular responses to calcium are modulated by a family of Ca²⁺/Calmodulin dependent protein kinases. The members of this family include CaMK I, CAMK II and CAMK IV. These kinases mediate rapid, highly specific and reversible post-translational modification involving ATP. CaMK II is abundantly expressed in mammalian brain, with highest expression in neurons of hippocampus and cerebral cortex, and plays critical role in synaptic transmission, memory, learning and behaviour. CaMK II family has four members, CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK IIα?and β?isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMK IIγ and δ isoforms are expressed in all tissues. CaMK II is autophosphorylated at α-Thr286 residue in response to changes in the concentration of intracellular calcium. This may prolong physiological responses to temporary changes in Ca²⁺ concentrations. CaMK II regulates synaptic signalling by phosphorylating glutamate receptors in the neurons. A distinct CaMK II has also been implicated in the process of smooth muscle contraction by phosphorylation of myosin light chain kinase.
This product reacts with the recombinant and native rat phosphorylated CaMK II α subunit only (50 kDa). It does not cross-react with the non-phosphorylated α subunit or with the 60 kDa β subunit in either phosphorylation state.

Immunogen

phosphorylated (at Thr²⁸⁶) synthetic phosphopeptide corresponding to amino acids of rat and mouse CaM kinase IIα subunit that contains threonine 286.

Application

Anti-phospho-CaM Kinase IIα (pThr²⁸⁶) antibody may be used in indirect immunofluorescence at a working dilution of 1:100 in rat hippocampal cells fixed with cold methanol. Detection by immunoblotting is possible at a working dilution of 1:1000.

Biochem/physiol Actions

The antibody reacts specifically with both the recombinant and native rat phosphorylated CaM (Ca²⁺/calmodulin) dependent kinase IIα subunit. It does not cross-react with the non-phosphorylated α subunit or with the β subunit (60 kDa) in either phosphorylation state. By immunofluorescence on rat hippocampal cells, the antibody labels cell somas and neuropil areas, while nuclei are only lightly stained.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C265	SAB4504356	SAB4301224
Sigma-Aldrich P247 Monoclonal Anti-phospho-CaM Kinase IIα (pThr²⁸⁶) antibody produced in mouse	Sigma-Aldrich C265 Monoclonal Anti-CaM Kinase IIα (CaMKIIα) antibody produced in mouse	Sigma-Aldrich SAB4504356 Anti-phospho-CaMK2 (pThr²⁸⁶) antibody produced in rabbit	Sigma-Aldrich SAB4301224 Anti-phospho-CaMK2α/β/δ (pThr³⁰⁵) antibody produced in rabbit
description clone 22B1, purified immunoglobulin, buffered aqueous solution	description clone 6G9, purified immunoglobulin, buffered aqueous solution	description affinity isolated antibody	description affinity isolated antibody
species reactivity rat, mouse	species reactivity rat, mouse	species reactivity rat, human, mouse	species reactivity human
Gene Information mouse ... Camk2a(12322) rat ... Camk2a(25400)	Gene Information rat ... Camk2a(25400)	Gene Information human ... CAMK2D(817)	Gene Information human ... CAMK2A(815), CAMK2B(816), CAMK2D(817)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
clone 22B1, monoclonal	clone 6G9, monoclonal	clone polyclonal	clone polyclonal
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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