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P3738

Protein Kinase G Iβ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

Synonym(s):

cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human

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Size/SKUAvailabilityPrice
5 μg
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$804.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
2.7.11.12 (BRENDA, IUBMB)
MDL number:
MFCD03458230
Specific activity:
≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells

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recombinant

expressed in baculovirus infected Sf9 cells

Quality Segment

assay

≥95% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

mol wt

76 kDa (monomer)

UniProt accession no.

relevant disease(s)

cancer

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKG1(5592)

Application

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

Biochem/physiol Actions

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

Physical form

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

Other Notes

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

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Protein Kinase G Iβ human ≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

Sigma-Aldrich

P3738

Protein Kinase G Iβ human

PKG1alpha active human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0371

PKG1alpha active human

Anti-PRKG1 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

HPA007699

Anti-PRKG1 antibody produced in rabbit

Guanosine 3′,5′-cyclic monophosphate ≥98% (HPLC), powder

Sigma-Aldrich

G7504

Guanosine 3′,5′-cyclic monophosphate

description

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

description

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

description

≥98% (HPLC), powder

assay

≥95% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

-

assay

≥98% (HPLC)

specific activity

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

specific activity

-

specific activity

-

specific activity

-

Gene Information

human ... PRKG1(5592)

Gene Information

human ... PRKG1(5592)

Gene Information

human ... PRKG1(5592)

Gene Information

-

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

-

recombinant

-

form

buffered aqueous glycerol solution

form

aqueous solution

form

buffered aqueous glycerol solution

form

powder

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

-

Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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