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Anti-Pyk2 antibody produced in rabbit

Name: Anti-Pyk2 antibody produced in rabbit
Brand: SIGMA
Price: 584 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CAK-β, Anti-Proline rich Kinase 2

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$584.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD01634069

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IHC (p), IP, WB

Citations:

$584.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 110-116 kDa

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections of human cerebellum., immunoprecipitation (IP): 3-5 μg/mL using 150-200 μg of PC-12 rat pheochromocytoma RIPA lysate, microarray: suitable, western blot: 1:2,000 using a whole extract of LPS-stimulated P388 mouse monocyte-macrophage cells

UniProt accession no.

Q14289

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PTK2B(2185)
mouse ... Ptk2b(19229)
rat ... Ptk2b(50646)

Description

General description

Pyk2 (proline-rich kinase 2) protein belongs to tyrosine protein kinase family and is primarily expressed in the central nervous system and in cells derived from hematopoietic lineages. Pyk2 is found in tissues and cells like mesenchymal, epithelial, endothelial cells, neonatal cardiomyocytes, osteoclasts and neuronal cells.

Immunogen

Synthetic peptide corresponding to amino acid residues 991-1009 of human Pyk2, coupled to KLH. This sequence is highly conserved in rat and mouse (1 amino acid substitution).

Application

Anti-Pyk2 antibody produced in rabbit has been used in:

immunocytochemistry
immunohistochemistry
western blotting

Biochem/physiol Actions

Protein tyrosine kinases (PTKs) are critical components of the signalling pathways that control cell growth, differentiation, apoptosis, metabolism, cell cycle regulation and cytoskeletal function. Pyk2 has been detected in cell-cell contacts, at focal adhesion-like structures and podosomes, cytoplasmic perinuclear region, in association with actin filaments and diffusely distributed in the cytoplasm. Pyk2 phosphorylation is critical for its interaction with SH2-containing signalling molecules and their linkage to signalling pathways that regulate extracellular-signal-regulated kinase (ERK), Janus kinases (JNK) and p38 kinases. Pyk2 has been shown to interact with Src family kinases, the growth factor receptor-bound protein 2 (Grb2)/Sos complex, p130cas, paxillin, Hic-5, and several other proteins, including inhibitors, to regulate signalling as well as cytoskeletal and morphological changes of cells. It has a crucial role in T and B cell antigen receptor signaling, cell cycle progression, metastasis, NK cytotoxicity, modulation of ion channel function and neuronal short- and long- term responses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4300468	SAB5700430	ZRB2135
Sigma-Aldrich P3902 Anti-Pyk2 antibody produced in rabbit	Sigma-Aldrich SAB4300468 Anti-PTK2B (Ab-402) antibody produced in rabbit	Sigma-Aldrich SAB5700430 Anti-Phospho-Pyk2-Y402 antibody produced in rabbit	Sigma-Aldrich ZRB2135 Anti-Pyk2/PTK2B Antibody, clone 3B7 ZooMAb^® Rabbit Monoclonal
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody	description -	description recombinant, expressed in HEK 293 cells
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified antibody
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
mol wt antigen 110-116 kDa	mol wt ~116 kDa	mol wt 115, 111	mol wt observed mol wt ~115 kDa, calculated mol wt 115.88 kDa
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form lyophilized

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c