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P4649
Phosphorylase b from rabbit muscle
For use as a marker in SDS-PAGE
Synonym(s):
α-Glucan Phosphorylase, 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase, Glycogen Phosphorylase
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Available to ship TODAYfromMILWAUKEE | $79.80 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-737-0
MDL number:
EC Number:
General description
Phosphorylase b is a dimer, usually exists in inactive form in the skeletal muscles. Equilibrium exists between an active relaxed (R) state and a less active tense (T). Phosphorylase b favors the T state. The enzyme possesses three domains, the N-terminal domain, glycogen-binding domain and the C-terminal domain.
Application
Phosphorylase b from rabbit muscle is to be used as a marker in SDS-PAGE. Phosphorylase b is used during chemical cross-linking studies as a SDS-PAGE molecular weight standard.
Phosphorylase b from rabbit muscle has been used as a molecular weight marker in 12% polyacrylamide gel for keratinase, and stress-70 protein.
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This Item | |||
|---|---|---|---|
| description For use as a marker in SDS-PAGE | description lyophilized powder, ≥20 units/mg protein, 2× crystallization | description lyophilized powder, 20-30 units/mg protein | description recombinant, expressed in E. coli, lyophilized powder |
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt observed mol wt ~97 kDa | mol wt 97,200 Da by calculation | mol wt - | mol wt 90 kDa by SDS-PAGE |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| packaging vial of 0.5 mg | packaging - | packaging - | packaging - |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Has the amino acid sequence of phosphorylase from rabbit muscle been published?
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The sequence has been published. The amino acid composition is:Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf).Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric form. The sequence information of the subunit would be the same for both forms.
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