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3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae)

Name: 3-Phosphoglyceric Phosphokinase from baker′s yeast (<I>S. cerevisiae</I>)
Brand: SIGMA
Price: 106 USD
Availability: InStock

ammonium sulfate suspension, ≥500 units/mg protein

Synonym(s):

PGK, Phosphoglycerate kinase, 3-Phosphoglycerate kinase, ATP:3-Phospho-D-glycerate 1-phosphotransferase

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Size/SKU	Availability	Price
2000 units	Estimated to ship onSeptember 08, 2026fromSAINT LOUIS	$106.00
5000 units	Estimated to ship onSeptember 08, 2026fromSAINT LOUIS	$213.00
10000 units	Estimated to ship onSeptember 08, 2026fromSAINT LOUIS	$369.00

About This Item

CAS Number:

9001-83-6

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-636-1

NACRES:

NA.54

MDL number:

MFCD00130301

EC Number:

2.7.2.3 (BRENDA, IUBMB)

Specific activity:

≥500 units/mg protein

Biological source:

bakers yeast

Concentration:

1.0-10.0 mg/mL

$106.00

Estimated to ship onSeptember 08, 2026Details

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Properties

biological source

bakers yeast

Quality Segment

200

form

ammonium sulfate suspension

specific activity

≥500 units/mg protein

storage condition

(Tightly closed)

concentration

1.0-10.0 mg/mL

foreign activity

Glyceraldehyde-3-phosphate dehydrogenase ≤0.1%

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Research area: Cell Signaling

Phosphoglycerate kinase (PGK), a glycolytic enzyme, is isolated from a broad variety of organisms. This typical hinge-bending monomeric enzyme is well-conserved among the three domains of life. The enzyme is made up of a single folded polypeptide chain that divides into two nearly identical domains, each linked by two -helices (-helices 7 and 14) and separated by a deep cleft. This arrangement gives the enzyme its distinctive bilobed structure.

Application

3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae) has been used:

to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase
in the coupled assay to measure the backward activity of purified rabbit skeletal muscle nicotinamide adenine dinucleotide (NAD⁺)-dependent glyceraldehyde3-phosphate dehydrogenase (GAPDH)
in the assay of glyceraldehyde-3-phosphate dehydrogenase

Biochem/physiol Actions

3-Phosphoglyceric phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate phosphokinase activity is essential for glycolysis and gluconeogenesis. Phosphoglyceratekinase (PGK) plays a vital role in the glycolytic pathway by catalyzing one of the two ATP-producing reactions. It converts 1,3-bisphosphoglycerate (1,3BPGA) to 3-phosphoglycerate (3PGA). Additionally, it takes part in the process of gluconeogenesis by catalyzing the opposite reaction to create 1,3BPGA and adenosine diphosphate (ADP). Due to its participation in numerous processes other than energy metabolisms, such as pathogenesis, interaction with nucleic acids, tumorigenesis progression, cell death, and viral replication, PGK is also known as a moonlighting protein.

Physical form

Crystalline suspension in 3.0 M (NH₄)₂SO₄ and 0.04 M tetrasodium pyrophosphate solution, pH 8.0

Analysis Note

Protein determined by TCA Biuret.

Other Notes

One unit will convert 1.0 μmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C.

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This Item	U8501	G7877	G3664
Sigma-Aldrich P7634 3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich U8501 Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast	Sigma-Aldrich G7877 Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich G3664 Glutathione Reductase from baker′s yeast (S. cerevisiae)
description ammonium sulfate suspension, ≥500 units/mg protein	description Type X, lyophilized powder, ≥40 units/mg protein	description Type VII, ammonium sulfate suspension, ≥200 units/mg protein	description ammonium sulfate suspension, 100-300 units/mg protein (biuret)
biological source bakers yeast	biological source bakers yeast	biological source bakers yeast	biological source -
specific activity ≥500 units/mg protein	specific activity ≥40 units/mg protein	specific activity ≥200 units/mg protein	specific activity 100-300 units/mg protein (biuret)
form ammonium sulfate suspension	form lyophilized powder	form ammonium sulfate suspension	form ammonium sulfate suspension
concentration 1.0-10.0 mg/mL	concentration -	concentration -	concentration -
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in -	shipped in -	shipped in -

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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