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Anti-phospho-PAK1/2/3 (pSer¹⁴¹) antibody produced in rabbit

Name: Anti-phospho-PAK1/2/3 (pSer<SUP>141</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 813 USD
Availability: InStock

affinity isolated antibody, buffered aqueous glycerol solution

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1 vial	Check Cart for Availability	$813.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD06411890

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

$813.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

western blot: 1:1,000 using Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation

UniProt accession no.

Q13153

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer141)

Gene Information

human ... PAK1(5058)

Description

General description

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of serine 141 (serine 144 for PAK1 and serine 139 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
The antibody detects human PAK1 (46% homologous), PAK2, and PAK3 (46% homologous). Other species have not been tested.

Immunogen

synthetic phosphopeptide derived from the region of human PAK2 that contains serine 141.

Application

A working dilution of 1:1000 is recommended for immunoblotting detection in Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation.

Biochem/physiol Actions

The antibody is highly selective for the phosphorylated form of PAK and can be used to detect an active form of PAK1, as well as PAK2 and PAK3.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	P3237	SAB4502065	WH0010298M1
Sigma-Aldrich P7871 Anti-phospho-PAK1/2/3 (pSer¹⁴¹) antibody produced in rabbit	Sigma-Aldrich P3237 Anti-phospho-PAK1 (pThr²¹²) antibody, Mouse monoclonal	Sigma-Aldrich SAB4502065 Anti-PAK1 antibody produced in rabbit	Sigma-Aldrich WH0010298M1 Monoclonal Anti-PAK4 antibody produced in mouse
description affinity isolated antibody, buffered aqueous glycerol solution	description clone PK-18, purified from hybridoma cell culture	description affinity isolated antibody	description clone 3F10, purified immunoglobulin, buffered aqueous solution
antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form purified immunoglobulin
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
biological source rabbit	biological source mouse	biological source rabbit	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity human	species reactivity human, mouse, rat	species reactivity rat, mouse, human	species reactivity human
technique(s) western blot: 1:1,000 using Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation	technique(s) microarray: suitable, western blot: 0.2-0.4 μg/mL using a whole extract of transfected and activated COS-7 (monkey kidney) cells expressing phosphorylated PAK1, immunocytochemistry: suitable, indirect ELISA: suitable	technique(s) immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000, immunohistochemistry: 1:50-1:100, ELISA: 1:1000	technique(s) western blot: 1-5 μg/mL, indirect ELISA: suitable

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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