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Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse

Name: Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse
Brand: SIGMA
Price: 563 USD
Availability: InStock

clone F4, ascites fluid

Synonym(s):

Anti-ABC20, Anti-CD243, Anti-CLCS, Anti-GP170, Anti-MDR1, Anti-P-GP, Anti-PGY1, Anti-p-170

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$563.00
0.2 mL	Available to ship TODAYfromMILWAUKEE	$693.00 $589.05

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00282355

Conjugate:

unconjugated

Clone:

F4, monoclonal

Application:

ELISA (i), ICC, IHC (f), IHC (p), IP, RIA, WB

Citations:

$563.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

F4, monoclonal

mol wt

antigen 170-180 kDa

contains

15 mM sodium azide

species reactivity

hamster, human

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, radioimmunoassay: suitable using cell-surface RIA, western blot: suitable

isotype

IgG1

UniProt accession no.

P08183

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCB1(5243)

Description

General description

The ABCB1 (ATP binding cassette subfamily B member 1) gene is mapped to human chromosome 7q21.12. It encodes for P-glycoprotein, which is a membrane bound drug transporter protein, belonging to the ATP-binding cassette (ABC) transporters family. The gene is considered to be highly polymorphic.

Immunogen

mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Application

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse has been used in western blot analysis and Immunohistochemistry.

Biochem/physiol Actions

Overexpression of ABCB1 (ATP binding cassette subfamily B member 1) gene is a major cause for multi-drug resistance, which makes chemotherapy challenging for the treatment of osteosarcoma. P-glycoprotein (P-gp) mediates the energy-dependent efflux of xenobiotic to the outside of plasma membrane from the inside of the cell , thereby affecting the process of absorption, distribution, and excretion of drugs. Cyclosporine, a calcineurin inhibitor serves as a substrate for P-gp.

The antibody recognizes an epitope located in the amino terminal half of P-glycoprotein (Pgp), at the third extracellular loop of the molecule. The epitope is resistant to formalin fixation and periodate oxidation. The antibody detects specifically human MDR1 P-glycoprotein, but does not appear to recognize the human MDR3 product, nor the mouse mdr1a, mdr1b or the mdr3 P-glycoprotein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M4308	M7786	C7464
Sigma-Aldrich P7965 Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse	Sigma-Aldrich M4308 Monoclonal Anti-MDM2 antibody produced in mouse	Sigma-Aldrich M7786 Monoclonal Anti-Myosin (Smooth) antibody produced in mouse	Sigma-Aldrich C7464 Monoclonal Anti-Cyclin D1 antibody produced in mouse
description clone F4, ascites fluid	description clone SMP14, ascites fluid	description clone hSM-V, ascites fluid	description clone DCS-6, ascites fluid
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... ABCB1(5243)	Gene Information human ... MDM2(4193) mouse ... Mdm2(17246) rat ... Mdm2(246362)	Gene Information human ... MYH11(4629)	Gene Information human ... CCND1(595) mouse ... Ccnd1(12443) rat ... Ccnd1(58919)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone F4, monoclonal	clone SMP14, monoclonal	clone hSM-V, monoclonal	clone DCS-6, monoclonal
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid
species reactivity hamster, human	species reactivity human, rat, mouse (weak)	species reactivity guinea pig, human, pig, canine, rabbit, chicken	species reactivity rat, mouse, human, monkey

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c