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P9290

Proteinase K–Agarose from Tritirachium album

lyophilized powder

Synonym(s):

Proteinase K-Agarose

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10 units
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$417.00
25 units

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$909.00

About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00132131
Form:
lyophilized powder
Biological source:
microbial (T.album
T. ALBUM)

$417.00

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biological source

microbial (T.album
T. ALBUM)

Quality Segment

200

form

lyophilized powder

extent of labeling

30-140 units per g solid

technique(s)

DNA extraction: suitable, RNA extraction: suitable, protein extraction: suitable

swelling

1 g swells to ~5 mL

storage temp.

−20°C

Application

Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.

Biochem/physiol Actions

The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.

Features and Benefits

• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.

Physical form

Lyophilized powder stabilized with lactose

Other Notes

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

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This Item
P2308P6556P8044
Proteinase K–Agarose from Tritirachium album lyophilized powder

Sigma-Aldrich

P9290

Proteinase K–Agarose from Tritirachium album

Proteinase K from Tritirachium album lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

Sigma-Aldrich

P2308

Proteinase K from Tritirachium album

Proteinase K from Tritirachium album lyophilized powder, ≥30 units/mg protein

Sigma-Aldrich

P6556

Proteinase K from Tritirachium album

Proteinase K from Tritirachium album ≥3.0 unit/mg solid, lyophilized powder

Sigma-Aldrich

P8044

Proteinase K from Tritirachium album

description

lyophilized powder

description

lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

description

lyophilized powder, ≥30 units/mg protein

description

≥3.0 unit/mg solid, lyophilized powder

biological source

microbial (T.album
T. ALBUM)

biological source

-

biological source

-

biological source

maize, soybean, yeast, plant seeds (barley), microbial (T.album
T. ALBUM)

technique(s)

protein extraction: suitable, DNA extraction: suitable, RNA extraction: suitable

technique(s)

-

technique(s)

DNA extraction: suitable

technique(s)

DNA extraction: suitable

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

400

Quality Level

300

Quality Level

200

swelling

1 g swells to ~5 mL

swelling

-

swelling

-

swelling

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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