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P9290

Proteinase K–Agarose from Tritirachium album

lyophilized powder

Synonym(s):

Proteinase K-Agarose

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10 units

Available to ship TODAYfromMILWAUKEE

$432.00
25 units

Available to ship TODAYfromMILWAUKEE

$942.00

About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00132131
Form:
lyophilized powder
Biological source:
microbial (T.album
T. ALBUM)

$432.00

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biological source

microbial (T.album
T. ALBUM)

Quality Segment

form

lyophilized powder

extent of labeling

30-140 units per g solid

technique(s)

DNA extraction: suitable, RNA extraction: suitable, protein extraction: suitable

swelling

1 g swells to ~5 mL

storage temp.

−20°C

Application

Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.

Biochem/physiol Actions

The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.

Features and Benefits

• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.

Physical form

Lyophilized powder stabilized with lactose

Other Notes

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

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This Item
SAE0009P5568SRE0005
Proteinase K–Agarose from Tritirachium album lyophilized powder

Sigma-Aldrich

P9290

Proteinase K–Agarose from Tritirachium album

Proteinase K from Tritirachium album ≥30 units/mg protein

Sigma-Aldrich

SAE0009

Proteinase K from Tritirachium album

Proteinase K from Tritirachium album ≥500 units/mL, buffered aqueous glycerol solution

Sigma-Aldrich

P5568

Proteinase K from Tritirachium album

Proteinase K from Tritirachium album Reagents designed and manufactured under current ISO 13485 certification.

Sigma-Aldrich

SRE0005

Proteinase K from Tritirachium album

description

lyophilized powder

description

≥30 units/mg protein

description

≥500 units/mL, buffered aqueous glycerol solution

description

Reagents designed and manufactured under current ISO 13485 certification.

technique(s)

protein extraction: suitable, DNA extraction: suitable, RNA extraction: suitable

technique(s)

DNA extraction: suitable

technique(s)

DNA extraction: suitable

technique(s)

DNA extraction: suitable, RNA extraction: suitable

biological source

microbial (T.album
T. ALBUM)

biological source

fungus (Tritirachium album)

biological source

microbial (T.album
T. ALBUM)

biological source

microbial

form

lyophilized powder

form

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

500

swelling

1 g swells to ~5 mL

swelling

-

swelling

-

swelling

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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