PCRISPR004
Mouse CRISPR Brie Knockout Library
Sinonimo/i:
CRISPR Brie Library, CRISPR Library for Mice, Mouse Knockout Library
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 ea | Per conoscere la disponibilità, visualizza il carrello | CHF 6610.00 |
Informazioni su questo articolo
NACRES:
NE.02
UNSPSC Code:
41105904
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartipackaging
pkg of 5 vials (5x200µL aliquots )
concentration
≥5x108 VP/ml (via p24 Assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Application
- Functional Genomics/Target Validation
- Unbiased wholed genome forward genetic screening
- Validated positive and negative controls
- Set up and optimization of screen assay
Biochem/physiol Actions
In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.
Features and Benefits
- Focus on your research, and we will generate your lentivirus screening library.
- Use CRISPR nucleases to knockout protein-coding genes to assess their function.
- Mouse Genes targeted 19,674
- Compact library of ~ four gRNAs per gene (78,637 total)
- Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9.
1 of 1
Questo articolo | |||
|---|---|---|---|
| concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) | concentration ≥5x108 VP/ml (via p24 Assay) |
| application(s) CRISPR | application(s) CRISPR | application(s) CRISPR | application(s) CRISPR |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 5 vials (5x200µL aliquots ) | packaging pkg of 10 vials (5x200µL aliquots of each component) |
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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