Key Documents

View All Documentation

Rabbit anti-BRD4 Antibody, Affinity Purified

Name: Rabbit anti-BRD4 Antibody, Affinity Purified
Brand: SIGMA
Price: 1120 USD
Availability: InStock

Powered by Bethyl Laboratories, Inc.

Synonym(s):

CAP, HUNK1, HUNKI, MCAP, Protein HUNK1, bromodomain-containing 4, chromosome-associated protein

Select a Size

Change View

Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$1,120.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, IP, WB

Citations:

$1,120.00

Check Cart for Availability

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

Quality Segment

100

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

concentration

1 mg/mL

technique(s)

immunohistochemistry: 1:1,000-1:5,000, immunoprecipitation (IP): 2-5 μg/mg, western blot: 1:2,000-1:10,000

accession no.

NP_490597.1

UniProt accession no.

O60885

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

rabbit ... BRD4(23476)

Description

General description

Bromodomain-containing protein 4 (BRD4) belongs to the bromodomain and extra-terminal (BET) family. The protein has two bromodomains (BD1 and BD2) connected by a long interdomain linker.

Immunogen

The epitope recognized by PLA0227 maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).

Biochem/physiol Actions

Bromodomain-containing protein 4 (BRD4) works as a reader of epigenetic marks and is involved in chromatin remodeling and transcriptional regulation. Therefore, it is a target for oncology, inflammation and cardiovascular disease. This gene is upregulated in hepatocellular carcinoma and is associated with poor prognosis. BRD4 is also associated with melanoma, hepatocellular carcinoma, multiple myeloma, Burkitt′s lymphoma, acute myeloid leukemia and breast cancer. BRD4 connects cell cycle and transcription, works as a scaffold for transcription factors and bookmarks active genes during mitosis. It interacts with acetylated histone-3 and histone-4 tails and helps in maintaining chromatin architecture. Also, it regulates transcription elongation via phosphorylation of RNA polymerase II.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	HPA061646	SAB1410426	HPA015055
Sigma-Aldrich PLA0227 Rabbit anti-BRD4 Antibody, Affinity Purified	Sigma-Aldrich HPA061646 Anti-BRD4 antibody produced in rabbit	Sigma-Aldrich SAB1410426 Anti-BRD4 antibody produced in rabbit	Sigma-Aldrich HPA015055 Anti-BRD4 antibody produced in rabbit
description Powered by Bethyl Laboratories, Inc.	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution	description purified immunoglobulin, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
antibody form affinity purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
species reactivity human, mouse	species reactivity human	species reactivity human	species reactivity human
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
technique(s) immunohistochemistry: 1:1,000-1:5,000, immunoprecipitation (IP): 2-5 μg/mg, western blot: 1:2,000-1:10,000	technique(s) immunohistochemistry: 1:50-1:200, immunofluorescence: 0.25-2 μg/mL, ChIP: 1—10 μg (per reaction)	technique(s) western blot: 1 μg/mL	technique(s) immunohistochemistry: 1:500-1:1000, immunofluorescence: 0.25-2 μg/mL

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.