Product R1153, RNase B Glycoprotein Standard, is extensively dialyzed against deionized water prior to lyophilization. It is essentially salt free.
R1153
RNase B Glycoprotein Standard from bovine pancreas
≥90% (SDS-PAGE), lyophilized powder
Synonym(s):
Ribonuclease B from bovine pancreas, RNase B
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| Size/SKU | Availability | Price |
|---|---|---|
500 μg | Check Cart for Availability | $402.00 |
1 mg | Check Cart for Availability | $659.00 |
About This Item
$402.00
Check Cart for Availability
Product Name
RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade
grade
Proteomics Grade
Quality Segment
assay
≥90% (SDS-PAGE)
form
lyophilized powder
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
Application
RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard.
Analysis Note
RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.
Other Notes
Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn34 at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man5-9GlcNAc2. Due to the heterogeneity in the glycosylation at Asn34, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.
1 of 1
This Item | |||
|---|---|---|---|
| description Proteomics Grade | description Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein | description Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein | description (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) |
| assay ≥90% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay - | assay - |
| grade Proteomics Grade | grade - | grade - | grade Molecular Biology |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form solid | form (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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