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Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

Name: Monoclonal Anti-Ryanodine Receptor antibody produced in mouse
Brand: SIGMA
Price: 809 USD
Availability: InStock

clone 34C, ascites fluid

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100 μL	Check Cart for Availability	$809.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00282461

Conjugate:

unconjugated

Clone:

34C, monoclonal

Application:

IF (d), IHC (f), IP, WB

Citations:

$809.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

34C, monoclonal

mol wt

antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms)

species reactivity

human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep

technique(s)

direct immunofluorescence: suitable, immunohistochemistry (frozen sections): 1:1,000, immunoprecipitation (IP): suitable, western blot: 1:5,000

isotype

IgG1

UniProt accession no.

P21817

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RYR1(6261), RYR2(6262)
mouse ... Ryr1(20190), Ryr2(20191)
rat ... Ryr1(114207), Ryr2(84025)

Description

General description

The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.

The ryanodine receptor (RyR) is the channel responsible for the release of Ca²⁺ from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca²⁺ regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Immunogen

partially purified chicken pectoral muscle ryanodine receptors (RyR)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells.

Physical form

Purified IgG1 antibody containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R128	O4886	D219
Sigma-Aldrich R129 Monoclonal Anti-Ryanodine Receptor antibody produced in mouse	Sigma-Aldrich R128 Monoclonal Anti-Ryanodine Receptor antibody produced in mouse	Sigma-Aldrich O4886 Monoclonal Anti-Opsin antibody produced in mouse	Sigma-Aldrich D219 Monoclonal Anti-Dihydropyridine Receptor (α₂ Subunit) antibody produced in mouse
description clone 34C, ascites fluid	description clone C3-33, ascites fluid	description clone RET-P1, ascites fluid	description clone 20A, ascites fluid, buffered aqueous solution
clone 34C, monoclonal	clone C3-33, monoclonal	clone RET-P1, monoclonal	clone 20A, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
Gene Information human ... RYR1(6261), RYR2(6262) mouse ... Ryr1(20190), Ryr2(20191) rat ... Ryr1(114207), Ryr2(84025)	Gene Information rat ... Ryr1(114207), Ryr2(84025)	Gene Information human ... RHO(6010) mouse ... Rho(212541) rat ... Rho(24717)	Gene Information mouse ... Cacna2d1(12293) rat ... Cacna2d1(25399)
species reactivity human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep	species reactivity canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human	species reactivity goldfish, mouse, tiger salamander, rat, amphibian, quail, dove, duck, rabbit, bovine, human, turtle	species reactivity rat, mouse, rabbit, guinea pig
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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