Skip to Content
MilliporeSigma

Skip To

R1755

Rhodamine B isothiocyanate

BioReagent, mixed isomers, suitable for protein labeling

Synonym(s):

RBITC

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
100 mg

Estimated to ship onNovember 09, 2026fromMILWAUKEE

$198.00
500 mg
Check Cart for Availability
$745.00
1 g
Check Cart for Availability
$1,140.00

About This Item

Empirical Formula (Hill Notation):
C29H30ClN3O3S
CAS Number:
Molecular Weight:
536.08
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352116
EC Number:
253-248-9
MDL number:

$198.00


Estimated to ship onNovember 09, 2026Details


​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


description

labeling efficiency with bovine albumin > 70%

Quality Segment

product line

BioReagent

form

powder

mol wt

536.08 g/mol

composition

9-[2-carboxy-5(or 6)-isothiocyanatophenyl]-3,6-bis(diethylamino)xanthylium chloride, ≤100%

color

purple to red-brown

solubility

methanol: 10 mg/mL (dark red to very dark red and dark purple to very dark purple)

fluorescence

λex 543 nm; λem 580 nm in methanol

suitability

suitable for protein labeling

storage temp.

−20°C

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1

InChI

1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H

InChI key

YVSWPCCVTYEEHG-UHFFFAOYSA-N

General description

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.

Application

Rhodamine B isothiocyanate is used for the following applications:
  • Rhodamine isothiocyanate labeling
  • Rhodamine-labeled synthetic APF
  • Preparations of protein and fluorescent conjugates
  • Fluorescent red dye

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
28392423414102558
description

BioReagent, mixed isomers, suitable for protein labeling

description

mixed isomers

description

Dye content 97 %

description

0.2% in isopropanol, for TLC derivatization

suitability

suitable for protein labeling

suitability

-

suitability

-

suitability

-

color

purple to red-brown

color

red to red-brown, purple to violet

color

-

color

-

fluorescence

λex 543 nm; λem 580 nm in methanol

fluorescence

-

fluorescence

-

fluorescence

-

form

powder

form

powder

form

solid

form

-

solubility

methanol: 10 mg/mL (dark red to very dark red and dark purple to very dark purple)

solubility

methanol: 10 mg/mL

solubility

ethanol: 1 mg/mL

solubility

-

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

-


Still not finding the right product?

Explore all of our products under Rhodamine B isothiocyanate


Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

  1. Hello, which form does this product come in? powder or liquid please. I would also like to know if it can be conjugated with chitosan polymers (nanomicelles)

    1 answer
    1. The product is supplied as a powder.
      Rhodamine B Isothiocyanate can be used for various applications. The only suitability test performed during quality control testing is for labelling efficiency with Bovine Serum Albumin. There is no suitability testing or protocol available for conjugating chitosan polymers. A search of the literature will be required to locate a protocol.

      Helpful?

Reviews

No rating value

Active Filters