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Monoclonal Anti-Rab9 antibody produced in mouse

Name: Monoclonal Anti-Rab9 antibody produced in mouse
Brand: SIGMA
Price: 521.9 USD
Availability: InStock

~2 mg/mL, clone Rab9-29, purified immunoglobulin, buffered aqueous solution

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$614.00 $521.90

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03097193

Conjugate:

unconjugated

Clone:

Rab9-29, monoclonal

Application:

ARR, ICC, IP, WB

Citations:

$521.90

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Properties

Quality Segment

200

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Rab9-29, monoclonal

form

buffered aqueous solution

mol wt

~23 kDa

species reactivity

human, rat, hamster, canine, mouse

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 2 μg/mL using total cell extract of HeLa cells.

isotype

IgG1

UniProt accession no.

P51151

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB9A(9367)
mouse ... Rab9(56382)
rat ... Rab9a(84589)

Description

General description

Monoclonal Anti-Rab9 (mouse IgG1 isotype) is derived from the hybridoma Rab9-29 produced by the fusion of mouse cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein Rab-9A (Rab9) is a member of the Rab family of small guanosine triphosphatases (GTPases). The gene Rab9 is mapped to human chromosome Xp22.2. Rab9 GTPase is localized predominantly to late endosomes.

Immunogen

synthetic peptide corresponding to amino acids 187-200 of human Rab9, conjugated to KLH.

Application

Monoclonal Anti-Rab9 antibody produced in mouse has been used in:

western blotting
enzyme-linked immunosorbent assay(ELISA)
immunoprecipitation
immunocytochemistry

Biochem/physiol Actions

Monoclonal Anti-Rab9 recognizes human, canine, rat, mouse and hamster Rab9 (approx. 23 kDa)

Ras-related protein Rab-9A (Rab9) guanosine triphosphatase (GTPase) is required for the transport of mannose 6-phosphate receptors (MRPs) from endosomes to the trans-Golgi network (TGN). Rab GTPases are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein tail-interacting protein of 47 kDa (TIP47). This interaction is required for Rab9 stability on late endosomes and may be crucial for vesicular transport in the virus life cycle.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R8779	R5530	R7904
Sigma-Aldrich R5404 Monoclonal Anti-Rab9 antibody produced in mouse	Sigma-Aldrich R8779 Anti-Rab7 antibody, Mouse monoclonal	$Anti-Rab8 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich R5530 Anti-Rab8 antibody produced in rabbit	Sigma-Aldrich R7904 Anti-Rab5 antibody, Mouse monoclonal
description ~2 mg/mL, clone Rab9-29, purified immunoglobulin, buffered aqueous solution	description ~2 mg/mL, clone Rab7-117, purified from hybridoma cell culture	description IgG fraction of antiserum, buffered aqueous solution	description clone Rab5-65, purified from hybridoma cell culture
clone Rab9-29, monoclonal	clone Rab7-117, monoclonal	clone polyclonal	clone Rab5-65, monoclonal
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form IgG fraction of antiserum	antibody form purified immunoglobulin
biological source mouse	biological source mouse	biological source rabbit	biological source mouse
Gene Information human ... RAB9A(9367) mouse ... Rab9(56382) rat ... Rab9a(84589)	Gene Information human ... RAB7A(7879) mouse ... Rab7(19349) rat ... Rab7a(29448)	Gene Information human ... RAB8A(4218) mouse ... Rab8a(17274) rat ... Rab8a(117103)	Gene Information human ... RAB5A(5868) mouse ... Rab5a(271457) rat ... Rab5a(64633)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity human, rat, hamster, canine, mouse	species reactivity bovine, human, monkey, mouse, chicken, rat	species reactivity mouse, human, rat	species reactivity human, cat, bovine, mouse, rat

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c