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R7131

RPMI 1640 Amino Acids Solution (50×)

Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Amino Acid Supplement

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Size/SKUAvailabilityPrice
100 mL

Available to ship TODAYfromMILWAUKEE

$45.10

About This Item

NACRES:
NA.75
UNSPSC Code:
12352209

$45.10


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Quality Segment

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Amino Acids Solution (50×) serves as an amino acid concentrate to supplement Roswell Park Memorial Institute medium (RPMI)-based mammalian cell culture media.

Application

RPMI 1640 Amino Acids Solution (50×) has been used as a supplement:
  • in M9 minimal medium to culture the bacterial strains, SX701 and JE116
  • to culture blood neutrophils from lactation cows in vitro
  • for amino acid stimulation in amino-acid-free Roswell Park Memorial Institute medium (RPMI) medium to grow HeLa cells


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This Item
R1780R7513R6504
description

Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture

description

With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

description

Modified, with sodium bicarbonate, without methionine, cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

liquid

form

liquid

form

liquid

form

powder

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-


Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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Questions

1–3 of 3 Questions  
  1. Does the Amino Acid Mixture contain any kind of buffer and if yes, what kind of buffer and which concentration? If no buffer is added, how is the pH of the solution adjusted to 10?

    1 answer
    1. This product is prepared in water and includes no added buffers or other medium components. The pH is adjusted to 10 using a sodium hydroxide base, in order to keep all of the amino acid constituents in solution. Please see the link below to review the product formulation:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/639/063/r7131for.pdf

      Helpful?

  2. Need to ask if this contain any kind of Folate or Folic Acid, if yes what are the concentrations?

    1 answer
    1. This product does not contain Folate or Folic Acid. Please see the link below to review the complete formulation of this and other Amino Acid solutions:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/639/063/r7131for.pdf

      Helpful?

  3. I would like the product information on R 7131. That is what are the concentratioin of each of the amino acids?

    1 answer
    1. The amino acids concentration can be located on the Product Information Sheet. This document can be downloaded from the 'DOCUMENTATION' section under 'More Documents':
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/639/063/r7131for.pdf

      Helpful?

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