R7647
Random Nonamers
for use as primers in cDNA synthesis, 50 μM in H2O
Synonym(s):
Oligodeoxynucleotides
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| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Check Cart for Availability | $130.00 |
About This Item
NACRES:
NA.31
UNSPSC Code:
41105500
Form:
liquid
Storage temp.:
−20°C
$130.00
Check Cart for Availability
grade
Molecular Biology
Quality Segment
form
liquid
usage
0.1 mL sufficient for 100 RT-PCR reactions
concentration
50 μM in H2O
shipped in
wet ice
storage temp.
−20°C
General description
Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers.
Application
Random Nonamers are suitable for:
- First-strand cDNA synthesis
- Preparing cDNA libraries (with oligo (dT) primers
- Prepare labeled DNA probes for hybridizations
Features and Benefits
- When preparing cDNA libraries or dealing with situations where specific primers are not suitable, the random nonamers can be combined with anchored oligo (dT)23 primers (Product No. O4387).
- In cases of incomplete or missing sequence information, or when specific primers are not effective, the random nonamers offer a solution for cDNA library preparation.
- The random nonamers′ priming capability at higher temperatures (up to 65°C) is reduced, ensuring that they do not interfere with polymerase chain reaction(PCR) following transcription.
- Randomnonamers can also be used to prepare labeled DNA probes for hybridizationstudiesRandom nonamers serve as alternatives to specific reverse transcription (RT) primers for first-strand synthesis, cDNA library construction, and other applications.
Other Notes
For laboratory use only. Not for drug, household, or other uses.
Random Nonamers are provided as a 50 uM solution in water.
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This Item | ||
|---|---|---|
| description for use as primers in cDNA synthesis, 50 μM in H2O | description certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland | description 98% |
| grade Molecular Biology | grade certified reference material | grade - |
| Quality Level 200 | Quality Level 300 | Quality Level 100 |
| form liquid | form - | form solid |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
| concentration 50 μM in H2O | concentration - | concentration - |
| shipped in wet ice | shipped in - | shipped in - |
Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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