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Anti-Rab5 antibody, Mouse monoclonal

Name: Anti-Rab5 antibody, Mouse monoclonal
Brand: SIGMA
Price: 468.35 USD
Availability: InStock

clone Rab5-65, purified from hybridoma cell culture

Synonym(s):

Anti-RAS-associated Protein RAB5A

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Size/SKU	Availability	Price
200 μL	Estimated to ship onAugust 31, 2026fromMILWAUKEE	$551.00 $468.35

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01322589

Conjugate:

unconjugated

Clone:

Rab5-65, monoclonal

Application:

ARR, ELISA (i), ICC, WB

Citations:

$468.35

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Rab5-65, monoclonal

form

buffered aqueous solution

mol wt

~24 kDa

species reactivity

human, cat, bovine, mouse, rat

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HeLa cells or NIH-3T3 or NRK, indirect ELISA: suitable, microarray: suitable, western blot: suitable

isotype

IgG3

UniProt accession no.

P20339

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)

Description

General description

Anti-Rab5 antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the hybridoma Rab5-65 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C-terminal region of human Rab5, conjugated to KLH. Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane.

Rab5 (Ras-related protein Rab-5A) is a small GTPase , that has three isoforms - Rab5A, Rab5B and Rab5C. It is upregulated in several secretory cells. It is located on human chromosome 3p24.

Immunogen

synthetic peptide corresponding to the C- terminal region of human Rab5, conjugated to KLH.

Application

Anti-Rab5 antibody, Mouse monoclonal has been used in:

enzyme linked immunosorbent assay (ELISA)
immunocytochemistry
co-immunoprecipitation assay
western blotting
immunofluorescence

Biochem/physiol Actions

Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and early endosome antigen 1(EEA1).

Rab5 (Ras-related protein Rab-5A) controls endosomal trafficking. Overexpression of Rab5A leads to metastasis and poor prediction in colorectal cancer patients. It modulates common Rab5 effectors to control the secretory pathways. It controls the homotypic integration between early endosomes and also the integration between endocytic vesicles and early endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB5700060	R5530	R4779
Sigma-Aldrich R7904 Anti-Rab5 antibody, Mouse monoclonal	Sigma-Aldrich SAB5700060 Anti-RAB5A antibody produced in rabbit	$Anti-Rab8 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich R5530 Anti-Rab8 antibody produced in rabbit	Sigma-Aldrich R4779 Anti-Rab7 antibody produced in rabbit
description clone Rab5-65, purified from hybridoma cell culture	description -	description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
Gene Information human ... RAB5A(5868) mouse ... Rab5a(271457) rat ... Rab5a(64633)	Gene Information human ... RAB5A(5868)	Gene Information human ... RAB8A(4218) mouse ... Rab8a(17274) rat ... Rab8a(117103)	Gene Information human ... RAB7A(7879) mouse ... Rab7(19349) rat ... Rab7a(29448)
clone Rab5-65, monoclonal	clone polyclonal	clone polyclonal	clone polyclonal
species reactivity human, cat, bovine, mouse, rat	species reactivity mouse, human, rat	species reactivity mouse, human, rat	species reactivity mouse, human, rat
biological source mouse	biological source rabbit	biological source rabbit	biological source rabbit
antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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