Skip to Content
MilliporeSigma

Skip To

R8000

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

partially purified powder, 0.01-0.1 unit/mg solid

Synonym(s):

3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
1 unit

Available to ship TODAYfromMILWAUKEE

$218.00
5 units

Available to ship TODAYfromMILWAUKEE

$827.00

About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
0.01-0.1 unit/mg solid
Biological source:
spinach

$218.00


Available to ship TODAYDetails


​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


biological source

spinach

Quality Segment

form

partially purified powder

specific activity

0.01-0.1 unit/mg solid

mol wt

557 kDa

storage temp.

−20°C

General description

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.
Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Application

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
  • as a test protein in pepsin digestion studies
  • as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
  • in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid

Biochem/physiol Actions

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.

Other Notes

One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
P9752F0628F3013
description

partially purified powder, 0.01-0.1 unit/mg solid

description

Type I, partially purified powder, ≥40 units/mg protein (biuret)

description

lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

description

lyophilized powder, Ferredoxin ≥15 wt. %

specific activity

0.01-0.1 unit/mg solid

specific activity

≥40 units/mg protein (biuret)

specific activity

≥15 units/mg solid

specific activity

-

biological source

spinach

biological source

spinach

biological source

-

biological source

spinach (Spinacia oleracea)

form

partially purified powder

form

partially purified powder

form

lyophilized powder

form

lyophilized powder

mol wt

557 kDa

mol wt

-

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library




Questions

Reviews

No rating value

Active Filters