Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

R8282

PRKG1, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

CGKI, DKFZp686K042, FLJ36117, MGC71944, PGK

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
10 μg
Check Cart for Availability
$433.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
663-897 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells

$433.00

Check Cart for Availability
​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in baculovirus infected Sf9 cells

Quality Segment

200

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

663-897 nmol/min·mg

mol wt

~100 kDa

UniProt accession no.

Q13976

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKG1(5592)

Biochem/physiol Actions

PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain. By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation. Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
SRP0371P3738A4611
PRKG1, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

R8282

PRKG1, active, GST tagged human

PKG1alpha active human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0371

PKG1alpha active human

Protein Kinase G Iβ human ≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

Sigma-Aldrich

P3738

Protein Kinase G Iβ human

AMPK (α1/β2/γ1), active, His tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

A4611

AMPK (α1/β2/γ1), active, His tagged human

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

description

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

specific activity

663-897 nmol/min·mg

specific activity

-

specific activity

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

specific activity

297-401 nmol/min·mg

Gene Information

human ... PRKG1(5592)

Gene Information

human ... PRKG1(5592)

Gene Information

human ... PRKG1(5592)

Gene Information

human ... PRKAA1(5562), PRKAB2(5565), PRKAG1(5571)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

form

buffered aqueous glycerol solution

form

aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

mol wt

~100 kDa

mol wt

80 kDa

mol wt

76 kDa (monomer)

mol wt

A1 subunit ~68 kDa, B2 subunit ~36 kDa, G1 subunit ~40 kDa

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters