The Positive Control functions as a system control to verify the functionality of the kit components. The resulting OD will not be utilized in any calculations. Any signal within the standard curve range that demonstrates competition in the assay indicates that the assay is performing as anticipated.
RAB0010
Angiotensin II EIA Kit
for serum, plasma, culture supernatant and cell lysates
Synonym(s):
Angiotensinogen, Serpin A8
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery) | $577.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352204
$577.00
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
species reactivity
mouse, human, rat
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, enzyme immunoassay: suitable
input
sample type cell lysate
sample type culture supernatant(s)
sample type serum
sample type plasma
assay range
inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 2.62 pg/mL
standard curve range: 0.1-1000 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... AGT(183)
General description
The Angiotensin II Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Angiotensin II peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANGII-1 can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV. However, it does not detect inactive angiotensinogen.
Immunogen
Angiotensin II, synthetic peptide
Application
Angiotensin II EIA Kit has been used for quantitative estimation of serum angiotensin-II.[1]
For research use only. Not for use in diagnostic procedures.
Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively.[1]
Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively.[1]
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Study on in vitro model shows that mechanical stretch induced Ang II release plays a vital role in mediating stretch-induced hypertrophic response. Ang II is implicated in maintaining blood pressure and has an important role in hypertension and in the pathophysiology of cardiovascular disorders. Ang II also has an essential role in growth induction, cell migration and mitosis of vascular smooth muscle cells. It enhances the production of collagen type I and III in fibroblasts, that results in thickening of the vascular wall and myocardium, and fibrosis. Variation in Ang II gene increases the risk of myocardial infarction, hypertension and left ventricular hypertrophy. Ang II expression is associated with heart failure, cardiac remodeling, diabetes, and the proliferative and inflammatory responses to arterial injury. The pharmacological agents such as angiotensin II-converting enzyme inhibitors or angiotensin II receptor antagonists can be used for treating Ang II-induced hypertension and congestive heart failure.
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This Item | |||
|---|---|---|---|
| Gene Information human ... AGT(183) | Gene Information human ... AGT(183) | Gene Information human ... NPPA(4878) | Gene Information human ... PNPLA3(80339) |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| species reactivity mouse, human, rat | species reactivity human | species reactivity mouse, human, rat | species reactivity rat, mouse, human |
| technique(s) ELISA: suitable, enzyme immunoassay: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable, enzyme immunoassay: suitable | technique(s) ELISA: suitable, enzyme immunoassay: suitable |
| assay range inter-assay cv: <15% | assay range inter-assay cv: <12% | assay range inter-assay cv: <15% | assay range inter-assay cv: <15% |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
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Related Content
Instructions
Autocrine release of angiotensin II mediates stretch-induced hypertrophy of cardiac myocytes in vitro.
Sadoshima J
Cell, 75(5), 977-984 (1993)
Genetic abrogation of adenosine A3 receptor prevents uninephrectomy and high salt-induced hypertension
Yang T, et al.
Journal of the American Heart Association, 5(7), e003868-e003868 (2016)
Role of angiotensin II in blood pressure regulation and in the pathophysiology of cardiovascular disorders.
Fyhrquist F
Journal of Human Hypertension, 9 suppl 5, S19-S24 (1995)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| RAB0010-1KT | 04061834959021 |
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What is the expected response for the positive control in the Human Angiotensin II EIA (RAB0010)?
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