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RAB0036

Human Carbonic Anhydrase IX ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

CA9

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1 kit
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
$567.00

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... CA9(768)
Input:
sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

$567.00

Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.2 pg/mL
standard curve range: 0.205-50 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CA9(768)

General description

Carbonic anhydrase IX (CA9) belongs to the zinc-containing metalloenzymes family. The protein is encoded by the gene mapped to human chromosome 9p. The encoded protein is localized on cell membrane and is characterized with an N-terminal proteoglycan-like domain (PG), a CA catalytic domain (CA), a transmembrane helical segment and a short intra-cytoplasmic tail. CA9 is expressed at very low levels in normal tissues (mostly in gastrointestinal tract), but it is expressed at high level in various cancer tissues, such as renal, lung, cervical, ovarian, esophageal and breast cancer.
The Human CA-IX (Carbonic Anhydrase IX) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-IX in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CA9

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Carbonic anhydrase IX (CA9) aids in reversible hydration of carbon dioxide to carbonic acid and bicarbonate. CA9 functions as a promising biomarker for diagnosing clear cell renal cell carcinoma (ccRCC), tumor hypoxia and prostate cancer (PC). In addition to pH control, CA9 also plays a vital role in cell proliferation, adhesion and tumor invasion.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Carbonic Anhydrase IX/XII Inhibitor The Carbonic Anhydrase IX/XII Inhibitor controls the biological activity of Carbonic Anhydrase IX/XII. This small molecule/inhibitor is primarily used for Cancer applications.

Sigma-Aldrich

215900

Carbonic Anhydrase IX/XII Inhibitor

description

for serum, plasma, cell culture supernatant and urine

description

IgG fraction of antiserum, buffered aqueous solution

description

-

description

The Carbonic Anhydrase IX/XII Inhibitor controls the biological activity of Carbonic Anhydrase IX/XII. This small molecule/inhibitor is primarily used for Cancer applications.

Gene Information

human ... CA9(768)

Gene Information

human ... CA9(768)

Gene Information

human ... CA9(768)

Gene Information

-

species reactivity

human

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

-

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.2 pg/mL
standard curve range: 0.205-50 pg/mL

assay range

-

assay range

-

assay range

-

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

input

-

input

-

input

-

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

indirect ELISA: 1:1000, western blot: 1:100-1:500

technique(s)

western blot: 1:500-1:2000, immunohistochemistry: 1:50-1:200

technique(s)

-

detection method

colorimetric

detection method

-

detection method

-

detection method

-

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0303D0345
0329K0345
0927J0345
SAMPLE
1026I0345

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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