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RAB0377

Visfatin EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonym(s):

Visfatin ELISA Kit

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1 kit
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
$577.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

$577.00


Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

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species reactivity

rat, human, mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

input

sample type serum
sample type culture supernatant(s)
sample type cell lysate
sample type plasma

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.778 ng/mL
standard curve range: 0.1-1000 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NAMPT(10135)

General description

The Visfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Visfatin peptide based on the principle of Competitive Enzyme Immunoassay.

Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.

Immunogen

Visfatin, synthetic peptide

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells. Visfatin regulates inflammation and insulin sensitivity.

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This Item
RAB0006RAB0415RAB0385
description

for serum, plasma, culture supernatant and cell lysates

description

for serum, plasma, culture supernatant and cell lysates

description

for serum, plasma, culture supernatant and cell lysates

description

for serum, plasma, culture supernatant and cell lysates

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

Gene Information

human ... NAMPT(10135)

Gene Information

human ... PNPLA3(80339)

Gene Information

human ... RETN(56729)

Gene Information

human ... NPPA(4878)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

species reactivity

rat, human, mouse

species reactivity

rat, mouse, human

species reactivity

mouse, rat, human

species reactivity

mouse, human, rat

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.778 ng/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 21 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 466 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

input

sample type serum
sample type culture supernatant(s)
sample type cell lysate
sample type plasma

input

sample type cell lysate
sample type plasma
sample type culture supernatant(s)
sample type serum

input

sample type cell lysate
sample type culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids



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