The capture and detection antibodies are mouse monoclonal. The immunogens are recombinant human Osteocalcin with glutathione s-transferase (GST). These detect the large NH2- terminal fragment and intact hOC. The specificity of activated vs. inactivated forms has not been determined.
RAB1073
Human Osteocalcin ELISA Kit
for cell culture supernatants, plasma, and serum samples
Synonym(s):
Osteocalcin
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| Size/SKU | Availability | Price |
|---|---|---|
96 wells | Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery) | $721.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... BGLAP(632)
Input:
sample type cell culture supernatant(s)
sample type serum sample(s)
sample type plasma
sample type serum sample(s)
sample type plasma
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
$721.00
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
input
sample type cell culture supernatant(s)
sample type serum sample(s)
sample type plasma
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.4 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... BGLAP(632)
General description
This ELISA antibody pair detects human Osteocalcin. Other species are not determined.
Osteocalcin (OC/OCN) is a bone-building protein. This γ-carboxylated protein is made up of 49 amino acids and is synthesized by osteoblasts, odontoblasts and hypertrophic chondrocytes. The bone γ-carboxyglutamate protein (BGLAP), that produces OCN is located on human chromosome 1q22.
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.
Please refer to the attached Protocolfor details.
Biochem/physiol Actions
Osteocalcin (OC/ OCN) plays a major role in the bone remodeling cycle, affecting bone mineralization. It is considered as a marker of bone formation. OCN guards against the progression of diabetes and the metabolic syndrome.
Other Notes
A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.
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This Item | |||
|---|---|---|---|
| description for cell culture supernatants, plasma, and serum samples | description for cell culture supernatants, plasma, and serum samples | description for cell culture supernatants, plasma, and serum samples | description - |
| Gene Information human ... BGLAP(632) | Gene Information human ... TYMP(1890) | Gene Information human ... L1CAM(3897) | Gene Information human ... THBS4(7060) |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type cell culture supernatant(s) | input sample type cell culture supernatant(s) | input sample type serum sample(s) | input sample type cell culture supernatant(s) |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable |
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Good day, I would like to know what molecule this kit detects, carboxylated osteocalcin or subcarboxylated osteocalcin?
1 answer-
Helpful?
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Does the RAB1073 Kit detect both activated and inactivated osteocalcin, or just one of them?
1 answer-
The Capture and Detection Antibodies are of the mouse monoclonal type. The immunogens are derived from recombinant human Osteocalcin with glutathione s-transferase. These antibodies are capable of detecting the large NH2-terminal fragment and intact hOC. However, the specificity of activated versus inactivated forms has not been tested.
Helpful?
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Does this Human Osteocalcin Kit measure total osteocalcin level, meaning the sum of undercarboxylated and carboxylated Osteocalcin? or a specific type?
1 answer-
We have not tested this ELISA kit for specificity of undercarboxylated and carboxylated Osteocalcin; here is information about capture and detection antibodies used in the kit:
Capture and Detection Antibodies are mouse monoclonal.
Immunogens: Recombinant human Osteocalcin with glutathione s-transferase.
Detects the large NH2- terminal fragment and intact human osteocalcin.
The specificity of activated vs. inactivated forms is not tested.Helpful?
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I am writing this message to ask for a detail protocol of RAB1073 (Human Osteocalcin ELISA). I have checked your website and I could not find a detail protocol to process my experiment. Could you please send me one PDF?
1 answer-
The protocol is attached to the lot-specific Certificate of Analysis.
Please see the sample Certificate below:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB1073_1221J2004.pdfHelpful?
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