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Anti-SmB antibody, Mouse Monoclonal

Name: Anti-SmB antibody, Mouse Monoclonal
Brand: SIGMA
Price: 600.1 USD
Availability: InStock

clone 12F5, purified from hybridoma cell culture

Synonym(s):

Anti-SNRPB1, Anti-Sm protein B, Anti-Sm-B, Anti-SmB/SmB, Anti-Small nuclear ribonucleoprotein associated protein B, Anti-snRNP-B

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$706.00 $600.10
200 μL	Available to ship TODAYfromMILWAUKEE	$748.00 $635.80

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

12F5, monoclonal

Application:

ELISA (i), ICC, IP, WB

Citations:

$600.10

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

12F5, monoclonal

mol wt

antigen ~25 kDa

species reactivity

human, mouse, canine

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.5-1 μg/mL using HeLa nuclear cell extract

isotype

IgG1

UniProt accession no.

P14678

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SNRPB(6628)
mouse ... Snrpb(20638)

Description

General description

Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein.

SNRPB (small nuclear ribonucleoprotein-associated protein B) is an important constituent of the spliceosome. It codes for SmB/B′ (small nuclear ribonucleoprotein polypeptides B and B1). It is located on human chromosome 20p13. SmB′/B are upregulated in all tissues excluding postnatal brain.

Immunogen

SmB recombinant protein.

Application

Monoclonal Anti-SmB antibody produced in mouse has been used in:
western blotting
enzyme-linked immunosorbent assay (ELISA)
immunoprecipitation
immunocytochemistry

Biochem/physiol Actions

SNRPB (small nuclear ribonucleoprotein-associated protein B) mutations results in cerebro-costo-mandibular syndrome. Downregulation of SNRPB affects the upregulation and processing of RNA splicing machinery components. It also plays a major role in RNA stability.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	SAB4200202	R5028	SAB4200188
Sigma-Aldrich S0698 Anti-SmB antibody, Mouse Monoclonal	Sigma-Aldrich SAB4200202 Anti- INI1/SNF5 antibody, Mouse monoclonal	Sigma-Aldrich R5028 Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse	Sigma-Aldrich SAB4200188 Anti-U1 snRNP C (U1C) antibody, Rat monoclonal
description clone 12F5, purified from hybridoma cell culture	description clone 2C2, purified from hybridoma cell culture	description clone 4F4, purified immunoglobulin, buffered aqueous solution	description clone 4H12, purified from hybridoma cell culture
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source rat
Gene Information human ... SNRPB(6628) mouse ... Snrpb(20638)	Gene Information human ... SMARCB1(6598)	Gene Information human ... HNRNPC(3183)	Gene Information human ... SNRPC(6631) mouse ... Snrpc(20630) rat ... LOC685273(685273)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity human, mouse, canine	species reactivity monkey, bovine, canine, mouse, rat, human	species reactivity monkey, human, chicken, hamster	species reactivity human, mouse, rat, hamster, monkey
clone 12F5, monoclonal	clone 2C2, monoclonal	clone 4F4, monoclonal	clone 4H12, monoclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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