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S1826

Sialic Acid Aldolase from Escherichia coli K12

recombinant, expressed in E. coli BL21, ≥3.0 units/mg protein

Synonym(s):

N-Acetylneuraminate lyase, N-Acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming)

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Size/SKUAvailabilityPrice
10 units
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$435.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥3.0 units/mg protein
Recombinant:
expressed in E. coli BL21

$435.00


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recombinant

expressed in E. coli BL21

Quality Segment

form

lyophilized powder

specific activity

≥3.0 units/mg protein

mol wt

33.4 kDa

shipped in

dry ice

storage temp.

−20°C

General description

Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens.

Application

Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes.

Physical form

Lyophilized powder containing Tris-HCl and NaCl

Analysis Note

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 7.5) containing Neu-5-Ac (10 mM) at 37 °C for 15 min and analyzed using capillary electrophoresis with UV detection at 200 nm.

Other Notes

One unit will catalyze the formation of 1.0 μmol Neu-5-Ac from Man-N-Ac and pyruvate per minute at 37°C at pH 8.0.

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This Item
A6680A6007T0910
description

recombinant, expressed in E. coli BL21, ≥3.0 units/mg protein

description

lyophilized powder, ≥20 units/mg protein (biuret)

description

soluble powder, 75-150 units/mg solid

description

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

specific activity

≥3.0 units/mg protein

specific activity

≥20 units/mg protein (biuret)

specific activity

75-150 units/mg solid

specific activity

≥3 units/mg protein

recombinant

expressed in E. coli BL21

recombinant

-

recombinant

-

recombinant

expressed in E. coli

form

lyophilized powder

form

lyophilized powder

form

soluble powder

form

essentially salt-free, lyophilized powder

mol wt

33.4 kDa

mol wt

~98 kDa

mol wt

-

mol wt

11.7 kDa

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


Pictograms

Exclamation mark

Signal Word

Warning

Hazard Codes

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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