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S2015

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Synonym(s):

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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1 L

Available to ship TODAYfromMILWAUKEE

$96.50

About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

$96.50

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grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Preparation Note

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

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SSPE Buffer 20× Concentrate for Northern and Southern blotting, solution

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SSPE Buffer 20× Concentrate

SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

Sigma-Aldrich

S6639

SSC Buffer 20× Concentrate

SSPE Buffer for Northern and Southern blotting, powder blend

Sigma-Aldrich

S8140

SSPE Buffer

Sigma-Aldrich

Sigma-Aldrich

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SSC Buffer, 20X Concentrate

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

form

solution

form

solution

form

powder blend

form

liquid

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

foreign activity

DNAse, none detected, Exonuclease, none detected, RNAse, none detected, NICKase, none detected, Endonuclease, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

-

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

-

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000528941
0000528941
0000504311
0000504311
0000446238

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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Warren P Pickering et al.
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Increased protein degradation during metabolic acidosis contributes to muscle wasting in uraemia. Adrenalectomy experiments in severely acidotic rats (arterial pH approximately 7.15) have shown that this is prevented in the absence of glucocorticoid. It should therefore be possible to block
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
Pierce SE, et al.
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Global Trade Item Number

SKUGTIN
S2015-1L04061836916527

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