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Anti-S-100 (β-Subunit) antibody, Mouse monoclonal

Name: Anti-S-100 (β-Subunit) antibody, Mouse monoclonal
Brand: SIGMA
Price: 441.15 USD
Availability: InStock

clone SH-B4, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse, Anti-NEF, Anti-S100-B

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$519.00 $441.15
0.2 mL	Available to ship TODAYfromMILWAUKEE	$552.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162834

Conjugate:

unconjugated

Clone:

SH-B4, monoclonal

Application:

ELISA (i), IHC (p), WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

(Solution in 0.01M phosphate buffered saline pH 7.4, containing 15mM sodium azide as a preservative)

antibody product type

primary antibodies

clone

SH-B4, monoclonal

contains

15 mM sodium azide

species reactivity

sheep, bovine, goat, pig, feline, rabbit, rat, canine, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (: suitable), indirect ELISA: suitable (:suitable), western blot: 1:100-1:200 ( (using S-100B Protein, Bovine Brain))

isotype

IgG1

UniProt accession no.

P04271

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... S100B(6285)
rat ... S100b(25742)

Description

General description

Monoclonal Anti-S-100 (b-Subunit) (mouse IgG1 isotype) is derived from the SH-B4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. S-100 is a set of small, highly acidic, thermolabile dimer proteins of ~20 kDa, widely distributed in different tissues.

S-100 (β-Subunit) is a calcium-binding protein, that belongs to S100 family of calcium-binding proteins. It is expressed in melanocytes. It is located on human chromosome 21q22.3.

Immunogen

bovine brain S-100b

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in immunocytochemistry and immunohistochemistry. It has also been used as a component in serum medium for 5-ethynyl-29-deoxyuridine (EdU), incorporation assay.

Biochem/physiol Actions

Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca²⁺-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues.

S-100 (β-Subunit) acts as a marker of melanocyte cytotoxicity. Reduced expression of S100B is observed in chronic liver diseases. It also acts as a serum marker in endocrine resistant breast cancer.

S-100 is considered to be a cell-growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	ABN59	WH0006282M1	HPA015768
Sigma-Aldrich S2657 Anti-S-100 (β-Subunit) antibody, Mouse monoclonal	Sigma-Aldrich ABN59 Anti-S100B Antibody	Sigma-Aldrich WH0006282M1 Monoclonal Anti-S100A11 antibody produced in mouse	Sigma-Aldrich HPA015768 Anti-S100B antibody produced in rabbit
description clone SH-B4, purified from hybridoma cell culture	description from rabbit, purified by affinity chromatography	description clone 2F4, purified immunoglobulin, buffered aqueous solution	description affinity isolated antibody, buffered aqueous glycerol solution
biological source mouse	biological source rabbit	biological source mouse	biological source rabbit
antibody form (Solution in 0.01M phosphate buffered saline pH 7.4, containing 15mM sodium azide as a preservative)	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
clone SH-B4, monoclonal	clone polyclonal	clone 2F4, monoclonal	clone polyclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity sheep, bovine, goat, pig, feline, rabbit, rat, canine, human	species reactivity human	species reactivity human	species reactivity human
Gene Information human ... S100B(6285) rat ... S100b(25742)	Gene Information rat ... S100B(25742)	Gene Information human ... S100A11(6282)	Gene Information human ... S100B(6285)

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

WGK

WGK 1

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