If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
S4521
Saponin from Quillaja sp.
Sapogenin content 20-35 %
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
10 g | Check Cart for Availability | $72.80 |
25 g | Check Cart for Availability | $147.00 |
About This Item
$72.80
description
non-ionic
Quality Segment
composition
Sapogenin content, 20-35%
technique(s)
HPLC: suitable, protein quantification: suitable
sulfated ash
≤10%
solubility
soluble (Water solubility: 2.000 g/l at 19,5 °C - OECD Test Guideline 105 - completely)
SMILES string
O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O[C@H]2[C@@H](O[C@@H]([C@H]([C@@H]2O)O[C@@H]8O[C@@H]([C@H]([C@@H]([C@H]8O)O)O)CO)C(=O)O)O[C@@H]3[C@@](C4[C@@](C5[C@]([C@@]6(C[C@H]([C@]7([C@H]([C@@H](C(CC7C6=CC5)(C)C)OC(=O)\C(=C/C)\C)OC(=O)C)CO)O)C)(CC4)C)(CC3)C
InChI
1S/C55H86O24/c1-10-23(2)46(71)79-43-44(72-24(3)60)55(22-59)26(17-50(43,4)5)25-11-12-30-51(6)15-14-32(52(7,21-58)29(51)13-16-53(30,8)54(25,9)18-31(55)61)75-49-41(77-48-38(67)36(65)34(63)28(20-57)74-48)39(68)40(42(78-49)45(69)70)76-47-37(66)35(64)33(62)27(1
InChI key
AXNVHPCVMSNXNP-ZELRDNAQSA-N
General description
Application
- to stain fertilized oocytes
- in the analysis of intracellular cytokine production
- to permeabilize dendritic cells
Biochem/physiol Actions
Preparation Note
1 of 1
This Item | |||
|---|---|---|---|
| description Sapogenin content 20-35 % | description Sapogenin content ≥10 % | description A glycoside used to permeabilize cellular membranes. Exhibits hemolytic activity. | description - |
| technique(s) protein quantification: suitable, HPLC: suitable | technique(s) HPLC: suitable, protein quantification: suitable | technique(s) - | technique(s) protein quantification: suitable, LC/MS: suitable, HPLC: suitable |
| solubility soluble (Water solubility: 2.000 g/l at 19,5 °C - OECD Test Guideline 105 - completely) | solubility - | solubility water: soluble | solubility - |
| description non-ionic | description non-ionic | description Merck USA index - 14, 8365 | description non-ionic |
| Quality Level 300 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| composition Sapogenin content, 20-35% | composition Sapogenin content, ≥10% | composition - | composition Sapogenin content, 20-35% |
| sulfated ash ≤10% | sulfated ash <20% | sulfated ash - | sulfated ash ≤10% |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Gloves
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How can I determine the shelf life / expiration / retest date of this product?
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Is this product suitable for downstream molecular tests if used in a lysis buffer for DNA extraction ?
1 answer-
This product has not been tested for suitability with any molecular tests after being used in a DNA lysis buffer. Validation by the intended user is necessary, as this has not been assessed. Researchers associated with Radboud University Medical Center have reported the usage of product 47036, or a molecular biology grade saponin, for bacterial DNA isolation prior to qPCR. For more details, refer to the citation below.
Optimized DNA isolation method for microbiome analysis of human tissues
Carlijn Bruggeling, Daniel R. Garza, Soumia Achouiti, Wouter Mes, Bas E. Dutilh, Annemarie Boleij
bioRxiv 2020.08.25.267641; doi: https://doi.org/10.1101/2020.08.25.267641Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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