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Anti-Sir2 (AS-16) antibody produced in rabbit

Name: Anti-Sir2 (AS-16) antibody produced in rabbit
Brand: SIGMA
Price: 576 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SIR2, Anti-SIR2L1, Anti-SIR2alpha

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$576.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03792039

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

$576.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~110 kDa

species reactivity

mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:50 using mouse NIH3T3 cells, microarray: suitable, western blot: 1:2,000 using mouse NIH/3T3 cells

UniProt accession no.

Q923E4

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Sirt1(93759)

Description

General description

Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity.

Immunogen

synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.

Application

Anti-Sir2 (AS-16) antibody produced in rabbit has been used in

western blotting
immunoprecipitation
antibody microarray

Biochem/physiol Actions

Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.

Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). Sir2 protein is an NAD-dependent histone deacetylase. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	ZRB07131	S5196	SAB5700606
Sigma-Aldrich S5313 Anti-Sir2 (AS-16) antibody produced in rabbit	Sigma-Aldrich ZRB07131 Anti-Sirt1 (Sir2) Antibody, clone 1G16, ZooMAb^® Rabbit Monoclonal	Sigma-Aldrich S5196 Anti-Sirt1 antibody, Mouse monoclonal	Sigma-Aldrich SAB5700606 Anti-SIRT1 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description recombinant, expressed in HEK 293 cells	description clone SIR11, purified from hybridoma cell culture	description -
antibody form affinity isolated antibody	antibody form purified antibody	antibody form purified immunoglobulin, purified from hybridoma cell culture	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit (recombinant)	biological source mouse	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
mol wt antigen ~110 kDa	mol wt observed mol wt ~110 kDa, calculated mol wt 80.32 kDa	mol wt antigen ~105 kDa	mol wt 81, 61
Gene Information mouse ... Sirt1(93759)	Gene Information mouse ... Sirt1; Sir2l1(93759)	Gene Information mouse ... Sirt1(93759)	Gene Information human ... SIRT1(23411)

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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