Key Documents

View All Documentation

Anti-Sir2 (AS-16) antibody produced in rabbit

Name: Anti-Sir2 (AS-16) antibody produced in rabbit
Brand: SIGMA
Price: 472.6 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SIR2, Anti-SIR2L1, Anti-SIR2alpha

Select a Size

Change View

Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$556.00 $472.60

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03792039

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

$472.60

List Price$556.00Save 15%

Web-Only Promotion

Check Cart for Availability

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~110 kDa

species reactivity

mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:50 using mouse NIH3T3 cells, microarray: suitable, western blot: 1:2,000 using mouse NIH/3T3 cells

UniProt accession no.

Q923E4

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Sirt1(93759)

Description

General description

Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity.

Immunogen

synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.

Application

Anti-Sir2 (AS-16) antibody produced in rabbit has been used in

western blotting
immunoprecipitation
antibody microarray

Biochem/physiol Actions

Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.

Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). Sir2 protein is an NAD-dependent histone deacetylase. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	S8447	S5196	S5947
Sigma-Aldrich S5313 Anti-Sir2 (AS-16) antibody produced in rabbit	Sigma-Aldrich S8447 Anti-Sirt2 antibody produced in rabbit	Sigma-Aldrich S5196 Anti-Sirt1 antibody, Mouse monoclonal	Sigma-Aldrich S5947 Anti-Sirt7 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description clone SIR11, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture, purified immunoglobulin	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
technique(s) indirect immunofluorescence: 1:50 using mouse NIH3T3 cells, western blot: 1:2,000 using mouse NIH/3T3 cells, microarray: suitable	technique(s) immunoprecipitation (IP): 2-5 μg using extract of COS7 cells or 293 cells expressing recombinant human SIRT2 protein., indirect immunofluorescence: 5-10 μg/mL using human HeLa cells., western blot: 1-2 μg/mL using whole extracts of mouse and rat brain.	technique(s) western blot: 1-2 μg/mL using total cell extract of C-2 cells, indirect ELISA: suitable, microarray: suitable	technique(s) indirect immunofluorescence: 2-4 μg/mL using human HEK-293T cells, western blot: 1-2 μg/mL using whole extracts of HEK-293T cells expressing human Sirt7, immunoprecipitation (IP): 2-4 μg using extracts of HEK-293T cells expressing human Sirt7
mol wt antigen ~110 kDa	mol wt antigen 37:43 kDa (rat), antigen 43 kDa (human)	mol wt antigen ~105 kDa	mol wt antigen ~45 kDa

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c