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Sephadex^® G-50

Name: Sephadex<SUP>®</SUP> G-50
Brand: SIGMA
Price: 334 USD
Availability: InStock

BioReagent, DNA grade, Molecular Biology, fine

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Size/SKU	Availability	Price
25 g	Available to ship TODAYfromMILWAUKEE	$334.00

About This Item

CAS Number:

9004-54-0

UNSPSC Code:

23151817

NACRES:

NA.52

MDL number:

MFCD23701222.obs1210407

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Properties

grade

Molecular Biology

Quality Segment

200

product line

BioReagent

form

beads

technique(s)

DNA purification: suitable

matrix

cross-linked dextran

swelling

1 g swells to 9-11 mL

bead diameter

20-80 μm

pore size

exclusion limit (20 bp dsDNA (rA)₂₀)

pH

2-10

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

Description

General description

Sephadex^® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Application

Sephadex^® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Features and Benefits

Excellent recovery
Minimal sample dilution
Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

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Show Differences

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This Item	G5050	G50150	S6022
Sigma-Aldrich S5897 Sephadex^® G-50	Sigma-Aldrich G5050 Sephadex^® G-50	Supelco G50150 Sephadex^® G-50	Sigma-Aldrich S6022 Sephadex^® G-50
description BioReagent, DNA grade, Molecular Biology, fine	description Superfine	description Medium	description BioReagent, DNA grade, Molecular Biology, medium
grade Molecular Biology	grade -	grade -	grade Molecular Biology
technique(s) DNA purification: suitable	technique(s) affinity chromatography: suitable	technique(s) LPLC: suitable	technique(s) -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form beads	form powder	form slurry	form beads
storage temp. room temp	storage temp. -	storage temp. -	storage temp. room temp
product line BioReagent	product line -	product line -	product line BioReagent

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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