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Anti-phospho-S6-Kinase (pThr⁴²¹/pSer⁴²⁴) antibody produced in rabbit

Name: Anti-phospho-S6-Kinase (pThr<SUP>421</SUP>/pSer<SUP>424</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 934 USD
Availability: InStock

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-phospho-p70^S6K (pThr⁴²¹/pSer⁴²⁴)

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0.1 mL	Check Cart for Availability	$934.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD01092451

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB CL

Citations:

$934.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

mouse, rat, human

technique(s)

immunoprecipitation (IP): 1:250, western blot (chemiluminescent): 1:1,000

UniProt accession no.

P23443

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer421/pSer424)

Gene Information

human ... RPS6KB1(6198)
mouse ... Rps6kb1(72508)
rat ... Rps6kb1(83840)

Description

General description

S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus, mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging.
Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.

Immunogen

synthetic double phospho-Thr⁴²¹/Ser⁴²⁴ peptide corresponding to residues around Thr⁴²¹/Ser⁴²⁴ of human p70^S6K.

Application

Anti-phospho-S6-kinase (p70S6K) may be used for immunoblotting at a working dilution of 1:1000 in serum-treated NIH-3T3 cells. For immunoprecipitation, the recommended dilultion is 1:250.

Biochem/physiol Actions

Does not react with non-phosphorylated S6 kinase (p70^S6K) or other phosphorylated protein kinases.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4503957	SAB5700568	SAB5700776
Sigma-Aldrich S6436 Anti-phospho-S6-Kinase (pThr⁴²¹/pSer⁴²⁴) antibody produced in rabbit	Sigma-Aldrich SAB4503957 Anti-phospho-p70 S6 Kinase (pThr³⁸⁹) antibody produced in rabbit	Sigma-Aldrich SAB5700568 Anti-Phospho-P70S6K1-T389 antibody produced in rabbit	Sigma-Aldrich SAB5700776 Anti-P70 S6K antibody produced in rabbit
description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody	description -	description -
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
UniProt accession no. P23443	UniProt accession no. P23443	UniProt accession no. P23443	UniProt accession no. P23443
technique(s) western blot (chemiluminescent): 1:1,000, immunoprecipitation (IP): 1:250	technique(s) immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000, ELISA: 1:1000, immunohistochemistry: 1:50-1:100	technique(s) western blot: 1:500-1:2000	technique(s) western blot: 1:500-1:2000, immunohistochemistry: 1:50-1:200, immunofluorescence: 1:50-1:200

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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